UCD Conway Institute of Biomolecular and Biomedical Research, School of Medicine and Medical Science, University College Dublin, Belfield, Dublin, Ireland; School of Medicine and Medical Science, University College Dublin, St. Vincent's University Hospital, Elm Park, Dublin, Ireland.
Proteomics Clin Appl. 2009 Sep;3(9):1044-51. doi: 10.1002/prca.200900032. Epub 2009 Aug 28.
Our knowledge of the complex bronchoalveolar lavage fluid (BALF) proteome has increased significantly over the last decade; but still, there remain many aspects of the BALF proteome that need characterization. Current proteomic methodologies resolve proteins within limited dynamic ranges: thereby, being limited in their ability to examine important areas of the BALF proteome, such as low molecular weight, low abundance proteins. To ensure proper coverage of these proteins in the BALF proteome, a refined 2-DE standard operation protocol is presented, highlighting important issues in sample collection, sample preparation, and 2-D DIGE analysis. It is hoped that this will help advance the field of BALF proteomics, BALFomics, which has lagged behind similar biofluids such as plasma and serum.
在过去的十年中，我们对复杂的支气管肺泡灌洗液（BALF）蛋白质组学的了解有了显著的提高；但是，BALF 蛋白质组学仍有许多方面需要进一步研究。目前的蛋白质组学方法可以在有限的动态范围内解析蛋白质：因此，它们在检查 BALF 蛋白质组学的重要领域方面能力有限，例如低分子量、低丰度的蛋白质。为了确保 BALF 蛋白质组学中这些蛋白质的适当覆盖，提出了一种经过改进的 2-DE 标准操作方案，突出了样品收集、样品制备和 2-D DIGE 分析中的重要问题。希望这将有助于推动 BALF 蛋白质组学领域的发展，该领域落后于类似的生物流体，如血浆和血清。
