Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanit, Rome, Italy.
Proteomics Clin Appl. 2010 Nov;4(10-11):839-46. doi: 10.1002/prca.201000032. Epub 2010 Oct 4.
the vitreous gel is a highly hydrated extracellular matrix containing many proteins. These proteins are likely accumulated in the vitreous by local secretion, filtration from the blood, or diffusion from the surrounding tissues and vasculature, and may be altered in disease state. In the last several years, several reports of large-scale profiling of vitreous proteins have been published; however, there is little information on the characterization of the phosphoproteome of vitreous. Here, we sought to identify phosphopeptides and their phosphorylation sites from vitreous.
we used titanium dioxide (TiO(2) ) to enrich phosphopeptides from vitreous and identified them by LC-MS/MS.
we identified 85 unique phosphopeptides and the phosphorylation sites from 44 proteins.
we present a method for characterization of phosphoproteome from vitreous samples using current MS technologies and yielded an initial assessment of the phosphoprotein/peptide content of human vitreous, thus providing important biological information toward further understanding of the post-translational modifications of vitreous proteins and their functional significance in disease.
玻璃体凝胶是一种高度水合的细胞外基质，含有许多蛋白质。这些蛋白质可能通过局部分泌、从血液中过滤或从周围组织和血管扩散积累在玻璃体内，并可能在疾病状态下发生改变。在过去的几年中，已经发表了几篇关于大规模玻璃体蛋白质谱分析的报告；然而，关于玻璃体磷酸蛋白质组的特征描述的信息很少。在这里，我们试图从玻璃体内鉴定磷酸肽及其磷酸化位点。
我们使用二氧化钛（TiO₂）从玻璃体内富集磷酸肽，并通过 LC-MS/MS 进行鉴定。
我们从 44 种蛋白质中鉴定出 85 个独特的磷酸肽和磷酸化位点。
我们提出了一种使用当前 MS 技术从玻璃体样品中鉴定磷酸蛋白质组的方法，并对人玻璃体中的磷酸蛋白/肽含量进行了初步评估，从而为进一步了解玻璃体蛋白质的翻译后修饰及其在疾病中的功能意义提供了重要的生物学信息。
