Groupe ADEN EA4311, CIC 0204, Institut hospitalo-universitaire de recherche biomédicale, IFRMP23, Université et CHU de Rouen, Rouen France.
Proteomics Clin Appl. 2010 Mar;4(3):325-36. doi: 10.1002/prca.200800175. Epub 2010 Jan 4.
Glutamine (Gln) has protective, anti-inflammatory effects in animal models and humans. Antioxidant nutrients may exert synergistic effects on intestinal functions. Therefore, these combined nutrients may have a therapeutic potential during intestinal inflammation. This study was designed to investigate in humans the effects of a supplement composed of Gln and high-dosed antioxidant micronutrients compared to isomolar Gln only, on duodenal proteome.
Enteral perfusion of Gln (0.8 mmol x kg(-1) x h(-1)) or supplement was performed in two groups of six healthy volunteers during 5 h before taking endoscopic duodenal biopsies. Protein expression was analyzed by 2-DE and the relevant proteins identified by MS/MS.
About 1500 protein spots were revealed in both supplement and Gln conditions. Comparative proteomics analysis indicated that 11 proteins were differentially and significantly (p≤0.05) expressed in response to the supplement. These proteins were essentially implicated in metabolism pathways, e.g. fatty acid binding protein-1 and 40S ribosomal protein SA expressions were downregulated while manganese superoxide dismutase and retinal dehydrogenase-1 expressions were upregulated.
This study provides new information on human duodenal proteome and its nutritional modulation, and supports further clinical investigations designed to evaluate the effects of Gln plus antioxidants during intestinal inflammation and cancer.
谷氨酰胺（Gln）在动物模型和人类中具有保护和抗炎作用。抗氧化营养素可能对肠道功能具有协同作用。因此，这些联合营养素在肠道炎症期间可能具有治疗潜力。本研究旨在研究由 Gln 和高剂量抗氧化微量营养素组成的补充剂与仅 Gln 相比，对十二指肠蛋白质组的影响。
在 5 小时内，将 Gln（0.8 mmol x kg(-1) x h(-1)）或补充剂以肠内灌注的方式输送到两组 6 名健康志愿者体内，然后进行内窥镜十二指肠活检。通过 2-DE 分析蛋白质表达，并通过 MS/MS 鉴定相关蛋白质。
在补充剂和 Gln 条件下都揭示了约 1500 个蛋白质斑点。比较蛋白质组学分析表明，11 种蛋白质对补充剂有差异和显著（p≤0.05）的表达。这些蛋白质主要参与代谢途径，例如脂肪酸结合蛋白-1 和 40S 核糖体蛋白 SA 的表达下调，而锰超氧化物歧化酶和视网膜脱氢酶-1 的表达上调。
本研究提供了有关人类十二指肠蛋白质组及其营养调节的新信息，并支持进一步的临床研究，旨在评估 Gln 加抗氧化剂在肠道炎症和癌症期间的作用。
