Department of Surgery, School of Medicine, Gyeongsang National Unniversity, Gyeongsang National University Hospital, Gyeongnam Regional Cancer Center, Jinju, South Korea.
Proteomics Clin Appl. 2010 May;4(5):528-37. doi: 10.1002/prca.200900063. Epub 2010 Feb 26.
The aim of this study is to identify the potential tumor markers that function in carcinogenesis and tumor progression, thus providing important diagnostic and prognostic information.
We performed 2-D gel electrophoresis and MALDI-TOF MS to investigate the differentially expressed proteins in 25 papillary thyroid carcinoma tissues. For validation of candidate proteins and investigation of clinical significance, we performed Western, Northern blot analysis and immunohistochemical staining.
Our proteomic analyses revealed significantly decreased annexin A3 expression in papillary thyroid carcinoma at both the protein and mRNA levels, compared with normal thyroid tissue. ANXA3 immunoreactivity was not significantly correlated with lymph node metastasis, multifocality, capsular invasion or perithyroidal extension in thyroid cancer. However, the tumor subgroup with a lymph node metastasis score of >3 displayed significantly lower ANXA3 expression than did subgroups with negative and ≤3 scores (p=0.001). Moreover, ANXA3 expression was markedly lower in large tumors (>1 cm in diameter) than in microcarcinomas (p=0.001).
Decreased expression of ANXA3 in papillary thyroid cancer supports the idea that ANXA3 may be an effective marker of microcarcinoma, and a negative predictor of papillary thyroid cancer progression.
本研究旨在鉴定在癌症发生和肿瘤进展中起作用的潜在肿瘤标志物，从而提供重要的诊断和预后信息。
我们通过二维凝胶电泳和 MALDI-TOF MS 来研究 25 例甲状腺乳头状癌组织中的差异表达蛋白。为了验证候选蛋白并研究其临床意义，我们进行了 Western blot、Northern blot 分析和免疫组织化学染色。
与正常甲状腺组织相比，我们的蛋白质组学分析显示在甲状腺乳头状癌中 annexin A3 的蛋白和 mRNA 水平均显著降低。在甲状腺癌中，ANXA3 免疫反应性与淋巴结转移、多灶性、包膜侵犯或甲状腺周围延伸均无显著相关性。然而，淋巴结转移评分>3 的肿瘤亚组的 ANXA3 表达明显低于评分阴性和≤3 的亚组（p=0.001）。此外，在大肿瘤（直径>1 厘米）中 ANXA3 的表达明显低于微癌（p=0.001）。
甲状腺乳头状癌中 ANXA3 的表达降低支持 ANXA3 可能是微癌的有效标志物、且是甲状腺乳头状癌进展的阴性预测因子的观点。
