Dai Huiqing, Chen Chengyu, Yang Bin
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2010 Sep;35(17):2296-302.
To investigate the AAPH scavenging activities of 22 flavonoids and phenolic acids and 9 extracts of Chinese materia medica.
The antioxidant activities of the samples were evaluated by an oxygen radical absorbance capacity method (ORAC), at the same time, the total contents of flavonoids and phenolic the 9 herb extracts were analyzed by Folin-Ciocalteu method, and the active components were qualitatively and quantitatively analyzed by an HPLC method.
It was found that the tea extract showed the strongest AAPH activity with the ORAC value of 4786.40 micromol x g(-1) whereas safflower demonstrated the weakest activity with the ORAC value of 784.04 micromol x g(-1). As for compounds, quercetin had the strongest AAPH activity with the ORAC value of 12.90 while ( - )-EGC had the weakest activity with the ORAC value of 2.47. A quantitative relationship was obtained to describe the AAPH scavenging activity of the herb extracts: Y = 1844.8 lnX-3577.5, r = 0.8675, where Y stands for the ORAC vaule, and X stands for the concentration of total phenolic acids.
Flavonoids and phenolic acids are the AAPH scavenging active ingredients in the Chinese herb extracts. It's a good way to study the antioxidant activity of Chinese herb extract and its chemical composition by combing ORAC method and HPLC method.
研究22种黄酮类化合物、酚酸类化合物及9种中药提取物对2,2'-偶氮二异丁基脒二盐酸盐（AAPH）的清除活性。
采用氧自由基吸收能力法（ORAC）评价样品的抗氧化活性，同时采用福林-酚法分析9种草药提取物中黄酮类化合物和酚类化合物的总含量，并用高效液相色谱法对活性成分进行定性和定量分析。
发现茶叶提取物对AAPH的清除活性最强，ORAC值为4786.40微摩尔×克-1，而红花的活性最弱，ORAC值为784.04微摩尔×克-1。就化合物而言，槲皮素对AAPH的清除活性最强，ORAC值为12.90，而（-）-表儿茶素的活性最弱，ORAC值为2.47。得到了一个定量关系来描述草药提取物对AAPH的清除活性：Y = 1844.8 lnX - 3577.5，r = 0.8675，其中Y代表ORAC值，X代表总酚酸的浓度。
黄酮类化合物和酚酸类化合物是中药提取物中清除AAPH的活性成分。将ORAC法和HPLC法结合起来研究中药提取物的抗氧化活性及其化学成分是一种很好的方法。
