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滋补类中药材的抗氧化活性及总酚含量

Antioxidative activities and the total phenolic contents of tonic Chinese medicinal herbs.

作者信息

Guo D-J, Cheng H-L, Chan S-W, Yu P H-F

机构信息

Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, Shenzhen, China.

出版信息

Inflammopharmacology. 2008 Oct;16(5):201-7. doi: 10.1007/s10787-008-8016-9.

Abstract

Chinese medicated diet is an everyday practice in China. In this study, 16 commonly used soup making tonic Chinese medicinal herbs were selected for antioxidative capacities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP), and the total phenolic contents of these herbal extracts were measured by the Folin-Ciocalteu method. It confirmed that drinking tonic soups could supplement total antioxidants intake. Amongst the tested herbal extracts, extracts of Canarium album Raeusch., Flos caryophylli and Fructus amomi were found to have the highest antioxidative activities in both DPPH and FRAP assays. Their antioxidative activities were comparable to ascorbic acid and butylated hydroxytoluene. Thus, these herbs are safe and inexpensive sources of natural antioxidants. A significant relationship between the antioxidative effects and total phenolic contents were found, indicating phenolic compounds are the major contributor of antioxidative capacities of these herbs. In addition, a strong correlation between DPPH assay and FRAP assay implied that antioxidants in these herbs were capable of scavenging free radicals and reducing oxidants.

摘要

中药食疗在中国是一种日常做法。在本研究中,通过1,1-二苯基-2-苦基肼(DPPH)自由基清除活性和铁还原抗氧化能力(FRAP),选择了16种常用的煲汤滋补中药材进行抗氧化能力研究,并采用福林-酚法测定了这些草药提取物的总酚含量。结果证实,饮用滋补汤可以补充总抗氧化剂的摄入量。在测试的草药提取物中,榄仁、丁香和砂仁的提取物在DPPH和FRAP试验中均具有最高的抗氧化活性。它们的抗氧化活性与抗坏血酸和丁基化羟基甲苯相当。因此,这些草药是安全且廉价的天然抗氧化剂来源。研究发现抗氧化作用与总酚含量之间存在显著关系,表明酚类化合物是这些草药抗氧化能力的主要贡献者。此外,DPPH试验和FRAP试验之间的强相关性表明,这些草药中的抗氧化剂能够清除自由基并还原氧化剂。

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