Na S M, Jia P X, Yu M X
Institute of Microbiology, Academia Sinica, Beijing.
Wei Sheng Wu Xue Bao. 1990 Apr;30(2):117-21.
Two lytic bacteriophages of Bacillus subtilis BF7658 were isolated. Their morphology, biological properties and physiochemical characteristics of their DNAs were compared. Electromicroscopic observation indicated that BS31 has a hexagonal head and contracted tail sheath, BS32 is a complex short-tailed phage and similar to phi 29 but differs from phi 29 in size and other properties. Two phages have a narrow spectrum of host range. The molecular weight of BS31 and BS32 DNAs are 62kb and 17kb respectively by EcoR1 endonuclease analysis. The G + C content of the DNAs are 45.7%(BS31) and 40.7%(BS32). Structural proteins of BS31 gave two major bands and at least ten minor ones; BS32 gave three major bands and six minor ones by SDS-polyacralamide gel electrophoresis.
分离出了枯草芽孢杆菌BF7658的两种裂解性噬菌体。比较了它们的形态、生物学特性以及DNA的理化特征。电镜观察表明，BS31具有六边形头部和收缩的尾鞘，BS32是一种复杂的短尾噬菌体，与φ29相似，但在大小和其他特性上与φ29不同。两种噬菌体的宿主范围较窄。通过EcoR1内切酶分析，BS31和BS32 DNA的分子量分别为62kb和17kb。DNA的G + C含量分别为45.7%（BS31）和40.7%（BS32）。通过SDS-聚丙烯酰胺凝胶电泳，BS31的结构蛋白产生两条主要条带和至少十条次要条带；BS32产生三条主要条带和六条次要条带。
