Novosel'tsev N N, Marchenkov V I, Sorokin V M, Kravtsov A N, Degtiarev B M
Mol Gen Mikrobiol Virusol. 1990 Aug(8):18-21.
The bacteriophage designated RD2 has been isolated from the sewage in Rostov-on-Don city and studied. The morphology of bacteriophage particles and the biological properties of the bacteriophage make it related to the plague bacteriophage isolated by D'Errel. The molecular masses of the compared bacteriophages are almost identical being 26.4 +/- 0.4 Md for RD2 and 24.7 +/- 0.2 Md for D'Errel bacteriophage. The DNAs of the bacteriophages share 80% of homology and possess 15 nonhomologous regions scattered along the genomes. The phages are serologically related. The DNAs of both bacteriophages give the similar pattern of hydrolysis by restriction endonuclease EcoRV, but have the different sensitivity to many other restriction endonucleases. The protein specter of bacteriophage RD2 contains 18 polypeptides (11 minor ones), while the one of D'Errel bacteriophage contains 7 polypeptides similar in molecular mass with the polypeptides of RD2. The bacteriophage RD2 cannot be considered one of the plague causative agents of bacteriophages since the region where it has been isolated has a long epidemiological and epizootical record of absence of plague.
从顿河畔罗斯托夫市的污水中分离并研究了名为RD2的噬菌体。噬菌体颗粒的形态和噬菌体的生物学特性使其与戴尔尔分离出的鼠疫噬菌体相关。所比较的噬菌体的分子量几乎相同，RD2为26.4±0.4兆道尔顿，戴尔尔噬菌体为24.7±0.2兆道尔顿。噬菌体的DNA具有80%的同源性，并且在基因组中分布着15个非同源区域。这些噬菌体在血清学上相关。两种噬菌体的DNA经限制性内切酶EcoRV水解后呈现相似的模式，但对许多其他限制性内切酶的敏感性不同。噬菌体RD2的蛋白质谱包含18种多肽（11种次要多肽），而戴尔尔噬菌体的蛋白质谱包含7种分子量与RD2多肽相似的多肽。由于噬菌体RD2的分离区域长期以来在流行病学和动物流行病学记录中都没有鼠疫，因此不能将其视为鼠疫致病噬菌体之一。
