Femenia F, Alogninouwa T, Perre J, Parodi A L
Laboratoire d'Histologie et Anatomie Pathologique, INRA, Ecole Nationale Vétérinaire, Maisons Alfort, France.
Zentralbl Veterinarmed A. 1990 Mar;37(2):81-94.
A histological and cytological study of stimulated and non-stimulated bovine lymph nodes was performed using immunocytochemistry and electron-microscopic techniques. Immunohistochemical staining using polyclonal antibodies revealed cytoplasmic immunoglobulins producing lymphoid cells. A protein S100 specific serum evidenced antigen presenting cells, macrophages and endothelial cells. In order to identify T lymphocytes, two monoclonal antibodies (M1 and M12) were used. The electron-microscopic study confirmed the localization and identification of different lymphoid and non-lymphoid cells of the lymph node. The techniques used in the present work seem to be suitable for investigating bovine lymph nodes and those with pathologic alterations.
利用免疫细胞化学和电子显微镜技术，对刺激和未刺激的牛淋巴结进行了组织学和细胞学研究。使用多克隆抗体进行免疫组织化学染色，显示出产生细胞质免疫球蛋白的淋巴细胞。一种蛋白S100特异性血清证实了抗原呈递细胞、巨噬细胞和内皮细胞的存在。为了识别T淋巴细胞，使用了两种单克隆抗体（M1和M12）。电子显微镜研究证实了淋巴结中不同淋巴细胞和非淋巴细胞的定位和识别。本研究中使用的技术似乎适用于研究牛淋巴结以及有病理改变的淋巴结。
