Departamento de Fisiologia, Universidade Federal de Sergipe, Campus Universitário Prof. Aloisio de Campos, Av. Marechal Rondom, s/n, CEP 49.100-000, São Cristóvão, SE, Brazil.
Z Naturforsch C J Biosci. 2010 Sep-Oct;65(9-10):551-61. doi: 10.1515/znc-2010-9-1004.
Physicochemical characterization and antinociceptive and anti-inflammatory activities of atranorin (AT) extracted from Cladina kalbii Ahti in formalin- and capsaicin-induced orofacial pain and anti-inflammatory tests in rodents were studied. Physicochemical characterization showed that AT has the general formula C19H18O8. Male Swiss mice were pretreated with AT (100, 200, and 400 mg/kg, i.p.), morphine (3 mg/kg, i.p.), or vehicle (0.9% saline with two drops of 0.2% Tween 80) before formalin (20 microl, 2%) or capsaicin (20 microl, 2.5 microg) were injected into the right vibrissa. Our results showed that i.p. treatment with AT displayed marked inhibitory effects in different orofacial pain tests in mice. AT (400 mg/kg, i.p.) was effective in reducing the nociceptive face-rubbing behavioural response in both phases of the formalin test, which was also naloxone-sensitive. Additionally, AT produced a significant antinociceptive effect at all doses in the capsaicin test. Such results were unlikely to be provoked by motor abnormality, since AT-treated mice exhibited no performance alteration on the rota rod apparatus. AT exhibited significant anti-inflammatory activity in the acute model of inflammation (leukocyte migration to the peritoneal cavity), carrageenan- and arachidonic acid-induced hind paw edema in rats. Additionally, AT exhibited a dose-dependent antioxidant activity in vitro, as assessed by total radical-trapping antioxidant parameter and total antioxidant reactivity assays. All these findings suggest that AT might represent an important tool for the management of orofacial pain and/or inflammatory disorders.
从 Cladina kalbii Ahti 中提取的地衣酸（AT）的理化特性，以及在福尔马林和辣椒素诱导的口腔疼痛和抗炎试验中对啮齿动物的镇痛和抗炎活性进行了研究。理化特性表明 AT 的通用公式为 C19H18O8。雄性瑞士小鼠先用 AT（100、200 和 400mg/kg，ip）、吗啡（3mg/kg，ip）或载体（0.9%生理盐水加 2 滴 0.2%吐温 80）预处理，然后将福尔马林（20 微升，2%）或辣椒素（20 微升，2.5 微克）注入右侧触须。我们的结果表明，ip 给予 AT 对不同的口腔疼痛试验有明显的抑制作用。AT（400mg/kg，ip）可有效减少福尔马林试验的两个阶段中的疼痛性面部摩擦行为反应，且对纳洛酮敏感。此外，AT 在辣椒素试验的所有剂量下均产生显著的镇痛作用。这些结果不太可能是由运动异常引起的，因为 AT 处理的小鼠在转棒装置上没有表现出行为改变。AT 在急性炎症模型（白细胞向腹腔迁移）、角叉菜胶和花生四烯酸诱导的大鼠后爪肿胀中表现出显著的抗炎活性。此外，AT 在体外表现出剂量依赖性的抗氧化活性，如总自由基捕获抗氧化参数和总抗氧化反应性测定所评估的。所有这些发现表明，AT 可能是管理口腔疼痛和/或炎症性疾病的重要工具。
