Departamento de Fisiologia, Universidade Federal de Sergipe-UFS, Av. Marechal Rondon, s/n, São Cristovão, SE 49100-000, Brazil.
J Nat Med. 2012 Jul;66(3):428-34. doi: 10.1007/s11418-011-0601-3. Epub 2011 Oct 22.
Lippia gracilis Schauer is an aromatic plant widely found in Northeastern Brazil. The leaf infusions or decoctions and alcoholic macerate are used for some inflammatory diseases and headache. This paper reports the isolation of naringenin by semi-preparative liquid chromatography from the methanolic extract of L. gracilis (ELg) and the evaluation of the analgesic and anti-inflammatory activities of this extract by measuring nociception through acetic acid, formalin, and hot-plate tests in carrageenan-induced inflammation in mice. Following oral administration, ELg (100, 200, and 400 mg/kg) significantly reduced the number of writhes in the writhing test and the time of paw licks in both phases of the formalin test when compared to the control group animals. Mice treated with ELg did not exhibit any behavioral alteration during the hot plate and rota-rod tests, suggesting non-participation of the supraspinal components in the modulation of pain by ELg and no motor abnormality. The oral administration of 400 mg/kg of ELg produced an anti-inflammatory effect on peritonitis induced by carrageenan. These effects can be associated with a decrease of inflammatory mediator synthesis by compounds of ELg, such as naringenin, which has anti-inflammatory action as already described.
甜叶菊 Schauer 是一种广泛存在于巴西东北部的芳香植物。其叶的浸剂或煎剂及酒精浸膏被用于治疗一些炎症性疾病和头痛。本研究报告了从甜叶菊（ELg）的甲醇提取物中通过半制备液相色谱分离得到柚皮素，并通过醋酸、福马林和热板试验测量角叉菜胶诱导的炎症中小鼠的伤害感受，评估该提取物的镇痛和抗炎活性。与对照组动物相比，ELg（100、200 和 400mg/kg）经口服给药后，在扭体试验中明显减少了扭体次数，在福马林试验的两个阶段中也减少了舔爪时间。用 ELg 处理的小鼠在热板和转棒试验中没有表现出任何行为改变，这表明 ELg 对疼痛的调制不涉及脊髓以上成分，也没有运动异常。ELg 口服 400mg/kg 对由角叉菜胶引起的腹膜炎具有抗炎作用。这些作用可能与 ELg 中的化合物，如柚皮素，降低了炎症介质的合成有关，柚皮素已被描述具有抗炎作用。
