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柑橘残渣分离物可提高雨生红球藻虾青素的产量。

Citrus residues isolates improve astaxanthin production by Xanthophyllomyces dendrorhous.

机构信息

School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.

出版信息

Z Naturforsch C J Biosci. 2010 Sep-Oct;65(9-10):594-8. doi: 10.1515/znc-2010-9-1010.

DOI:10.1515/znc-2010-9-1010
PMID:21138061
Abstract

The wild strain and two astaxanthin-overproducing mutant strains, W618 and GNG274, of Xanthophyllomyces dendrorhous were analyzed in order to assess their ability to grow and synthesize astaxanthin in a minimal medium containing (per liter): 2 g KH2PO4, 0.5 g MgSO4, 2 g KNO3, and 1 g yeast extract, and supplemented with citrus residues isolates as a carbon source (citrus medium). The selected strain W618 was evaluated under various contents of citrus juice. At the content of 20% (v/v), the highest astaxanthin production reached 22.63 mg L(-1), which was two-fold more than that observed in yeast malt medium. Addition of 8% (v/v) n-hexadecane to the citrus medium was found to be optimal, increasing the astaxanthin yield by 21.7%.

摘要

为了评估球红酵母野生株和两株虾青素高产突变株 W618 和 GNG274 在含有(每升):2 g KH2PO4、0.5 g MgSO4、2 g KNO3 和 1 g 酵母提取物的最小培养基中生长和合成虾青素的能力,并补充柑橘残渣作为碳源(柑橘培养基)。选择的菌株 W618 在不同含量的柑橘汁下进行了评估。在 20%(v/v)的含量下,虾青素的最高产量达到 22.63 mg L(-1),比在酵母麦芽培养基中观察到的产量高出两倍。向柑橘培养基中添加 8%(v/v)正十六烷被发现是最佳的,虾青素的产量提高了 21.7%。

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