Evidence for lectin-mediated adherence of S. saprophyticus and P. aeruginosa to polymers.

By hemagglutination tests surface lectins on S. saprophyticus strain S 1 with N-acetylgalactosamine (GalNac) and N-acetylglucosamine (GlcNac) specificity and on P. aeruginosa ATCC strain 27853 with N-acetylneuraminic acid (NANA) specificity could be demonstrated. To elucidate the role of bacterial surface lectins for the specific adhesion, polyether urethane discs were preincubated for 15 h at 4 degrees C in human serum or urine. Adhesion studies with S. saprophyticus S1 and P. aeruginosa ATCC 27853 onto precoated polymers revealed that microbial lectins may play a role in the colonization of prosthetic devices since lectin-blocking with competitive glycoconjugates significantly decreased bacterial adherence to the coated surfaces. Non-specific carbohydrates did not inhibit the adherence demonstrating specificity of this process.