Food Safety Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.
J Agric Food Chem. 2011 Jan 12;59(1):78-84. doi: 10.1021/jf103439c. Epub 2010 Dec 7.
Thermoluminescence (TL) and 2-alkylcyclobutanone (2-ACB) analyses were performed to identify irradiated prawns ( Penaeus monodon ). With the TL method, minerals were extracted from prawns using acid hydrolysis. The experimental results satisfied the evaluation criteria of European Norm (EN) 1788, even after low-dose irradiation (0.5 kGy) and a 60 day storage at -20 °C. With the 2-ACB method, 2-dodecylcyclobutanone (2-DCB) and 2-tetradecylcyclobutanone (2-TCB) were successfully extracted from prawns by direct solvent extraction with purification using a conventional silica column and a sulfoxide column, which was used for 2-ACB for the first time. Both 2-ACB derivatives were absent from the non-irradiated samples but were identified in all irradiated samples by gas chromatography-mass spectrometry. Moreover, 2-DCB and 2-TCB production correlated with the applied dose (2.5-10 kGy), and the correlation did not diminish after 60 days of storage at -20 °C for any dose. Therefore, these two techniques provide rapid, simple, and promising methods for routine investigation of frozen prawns.
热释光（TL）和 2-烷基环丁酮（2-ACB）分析被用于鉴定辐照对虾（Penaeus monodon）。采用 TL 法，通过酸水解从对虾中提取矿物质。即使在低剂量辐照（0.5 kGy）和 -20°C 下储存 60 天后，实验结果仍满足欧洲标准（EN）1788 的评估标准。采用 2-ACB 法，通过直接溶剂萃取并用常规硅胶柱和亚砜柱进行纯化，成功地从对虾中提取了 2-十二烷基环丁酮（2-DCB）和 2-十四烷基环丁酮（2-TCB），这是首次将亚砜柱用于 2-ACB。未辐照样品中不存在 2-ACB 衍生物，但在所有辐照样品中均通过气相色谱-质谱法鉴定。此外，2-DCB 和 2-TCB 的生成与所施加的剂量（2.5-10 kGy）相关，并且在任何剂量下在 -20°C 下储存 60 天后，相关性并未减弱。因此，这两种技术为冷冻对虾的常规研究提供了快速、简单且有前途的方法。
