嘌呤2'-脱氧核糖核苷的酶促合成及其作为小牛肠黏膜和蜡样芽孢杆菌腺苷脱氨酶抑制剂的性质
Enzymatic synthesis of purine 2'-deoxyriboside and its properties as an inhibitor of adenosine deaminases from calf intestinal mucosa and Bacillus cereus.
作者信息
Caffaz S, Allegrini S, Cercignani G
机构信息
Dipartimento di Fisiologia e Biochimica, Laboratori di Biochimica, Università, Pisa.
出版信息
Ital J Biochem. 1990 Mar-Apr;39(2):115-20.
Enzymatic synthesis of purine 2'-deoxyriboside was obtained by reacting purine with excess 2-deoxy-alpha-D-ribose-1-phosphate in the presence of commercial bovine nucleoside phosphorylase; the product was isolated by semipreparative reverse phase HPLC with an overall 62% yield. Purine 2'-deoxyriboside was shown to behave as a competitive inhibitor of adenosine deaminase from calf intestinal mucosa and Bacillus cereus, with apparent Ki values of 4.5 and 8.5 microM, respectively.
在市售牛核苷磷酸化酶存在的情况下，嘌呤与过量的2-脱氧-α-D-核糖-1-磷酸反应，实现了嘌呤2'-脱氧核糖苷的酶促合成；通过半制备反相高效液相色谱法分离产物，总产率为62%。嘌呤2'-脱氧核糖苷被证明是来自小牛肠黏膜和蜡状芽孢杆菌的腺苷脱氨酶的竞争性抑制剂，其表观Ki值分别为4.5和8.5微摩尔。
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