[金黄色葡萄球菌肠毒素A基因在异源系统中的表达]
[Expression of Staphylococcus aureus enterotoxin A gene in heterologous systems].
作者信息
Chikindas M L, Isaeva S V, Chistiakova L G, Klinskiĭ E Iu, Grabovetskiĭ V V, Vasilenko O V, Sveshnikov P G, Alakhov V Iu, Kiselev V I
出版信息
Mol Gen Mikrobiol Virusol. 1990 Mar(3):21-4.
PMID:2113994
Abstract
The genomic library of Staphylococcus aureus genes on the plasmid vector pSL5 has been constructed. The library contains a 2.5 kb HindIII DNA fragment including the gene for enterotoxin A. The entA gene on the high copy number plasmids in the Escherichia coli cells deficient in proteolysis determines the synthesis of enterotoxin A in the amounts comparable to the ones in the parent strain Staphylococcus aureus FRI 722(H).
摘要
已构建了金黄色葡萄球菌基因在质粒载体pSL5上的基因组文库。该文库包含一个2.5kb的HindIII DNA片段，其中包括肠毒素A基因。在缺乏蛋白水解作用的大肠杆菌细胞中，高拷贝数质粒上的entA基因决定了肠毒素A的合成量，其合成量与亲本菌株金黄色葡萄球菌FRI 722(H)中的合成量相当。
Zotero 插件