Bond University, Gold Coast, Australia.
Eur J Appl Physiol. 2011 Jul;111(7):1307-12. doi: 10.1007/s00421-010-1756-4. Epub 2010 Dec 8.
Growth hormone (GH) is a commonly used drug aimed at improving sport performance. The aim of this study is to evaluate the immunomodulatory effects of short-term administration of recombinant GH (rhGH) in healthy young males. NK cell number, activity and phenotype, T cell number, CD4(+) (Th1/Th2) cytokine production of IL2, IL4, IL6, IL10, TNF-α and IFN-γ and CD4(+)/CD8(+) ratio with particular attention to the possible correlation to IGF-I production were investigated. 30 males (27 ± 9 years) were randomly assigned to placebo (n = 15) or drug (rhGH) 1 mg/day groups (n = 15) with daily injection for 7 days. IGF-I plasma concentration and flow cytometry data were generated at baseline and days 8, 15, 22 and 29 post injection. Data analysis used General Linear Model with repeated measures, Bonferroni correction factor and significance at p ≤ 0.05. Serum IGF-I levels (ng/mL) increased significantly (p ≤ 0.01) on day 8 (0.48 ± 0.78) after injections compared to baseline (0.31 ± 0.07) and days 15 (0.33 ± 0.06), 22 (0.29 ± 0.05) and 29 (0.29 ± 0.06). A significant time effect was noted in IL10 secretion (pg/mL) from day 15 (P = 35.14 ± 19.93, rhGH = 26.63 ± 16.39) to days 22 (P = 61.32 ± 20.41, rhGH = 74.99 ± 46.91) and 29 (P = 101.98 ± 67.25, rhGH = 107.74; ± 122.58). There was no correlation between IGF-I and NK activity, phenotype or number along with T lymphocyte number, CD4(+)/CD8(+) ratio or Th1 and Th2 cytokine production. In conclusion, cytokine secretion spectrum was not affected by short-term rhGH administration in young males.
生长激素（GH）是一种常用于提高运动表现的药物。本研究旨在评估短期给予重组生长激素（rhGH）对健康年轻男性的免疫调节作用。研究了 NK 细胞数量、活性和表型、T 细胞数量、CD4（+）（Th1/Th2）细胞因子产生的白细胞介素 2（IL2）、白细胞介素 4（IL4）、白细胞介素 6（IL6）、白细胞介素 10（IL10）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）以及 CD4（+）/CD8（+）比值，并特别关注 IGF-I 产生的可能相关性。将 30 名男性（27 ± 9 岁）随机分为安慰剂（n = 15）或药物（rhGH）1 mg/天组（n = 15），每天注射 7 天。在基线和注射后第 8、15、22 和 29 天采集 IGF-I 血浆浓度和流式细胞术数据。数据分析采用重复测量的一般线性模型，采用 Bonferroni 校正因子，p ≤ 0.05 为差异有统计学意义。与基线（0.31 ± 0.07）和第 15 天（0.33 ± 0.06）、第 22 天（0.29 ± 0.05）和第 29 天（0.29 ± 0.06）相比，注射后第 8 天（0.48 ± 0.78）血清 IGF-I 水平（ng/mL）显著升高（p ≤ 0.01）。第 15 天（P = 35.14 ± 19.93，rhGH = 26.63 ± 16.39）至第 22 天（P = 61.32 ± 20.41，rhGH = 74.99 ± 46.91）和第 29 天（P = 101.98 ± 67.25，rhGH = 107.74；± 122.58）时，IL10 分泌（pg/mL）存在显著的时间效应。IGF-I 与 NK 活性、表型或数量以及 T 淋巴细胞数量、CD4（+）/CD8（+）比值或 Th1 和 Th2 细胞因子产生之间无相关性。总之，短期 rhGH 给药并未影响年轻男性的细胞因子分泌谱。
