Wu Yajun, Chen Ying, Wang Bin, Gao Yunhua, Bai Liqun, Wang Haiyan
Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North St, Chaoyang District, Beijing, 100123, People's Republic of China.
J AOAC Int. 2010 Sep-Oct;93(5):1530-6.
Celery was found to provoke human allergenic response in some countries. Labeling of celery ingredients was required by the European Union, and the threshold set at 10 mg/kg (0.001%). In our study, a celery mannitol transporter (Mat3) gene-based detection method was established by means of SYBR Green real-time PCR technique. No cross-reactivity was found between celery and the other food materials. Absolute detection limit (LODa), relative detection limit (LODr), and practical detection limit (LODp) of the method were determined through experiments on pure celery DNA, DNA mix, and spiked food samples. The method was able to detect 0.001% raw food sample and 0.01% heated food sample. The utility of the method was confirmed by the investigation of 13 commercial foods.
在一些国家，芹菜被发现会引发人体过敏反应。欧盟要求对芹菜成分进行标注，设定的阈值为10毫克/千克（0.001%）。在我们的研究中，利用SYBR Green实时荧光定量PCR技术建立了一种基于芹菜甘露醇转运体（Mat3）基因的检测方法。未发现芹菜与其他食品原料之间存在交叉反应。通过对纯芹菜DNA、DNA混合物和加标食品样品进行实验，确定了该方法的绝对检测限（LODa）、相对检测限（LODr）和实际检测限（LODp）。该方法能够检测出0.001%的生食样品和0.01%的加热食品样品。通过对13种市售食品的检测，证实了该方法的实用性。
