特发性血小板减少性紫癜患者CD4+CD25+调节性T细胞及转化生长因子-β1的表达
[Expression of CD4+ CD25+ regulatory T cells and TGF-ß1 in patient with idiopathic thrombocytopenic purpura].
作者信息
Abudureheman Ayimunisa, Yasen Halida, Fang Zhao, Xue Zhang, Jian-bing Ding, Xiu-min Ma, Xin-hong Guo
机构信息
Department of Hematology, The First Affiliated Hospital of Xinjiang Medical University, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Sep;26(9):895-7.
AIM
To investigate the expression rate of CD4+ CD25+ regulatory T cells and TGF-ß1 in peripheral blood of the patients with idiopathic thrombocytopenic purpura (ITP), and the role they play in the pathogenesis of ITP.
METHOD
The population of CD4+ CD25+ regulatory T cells in peripheral blood of 31 patients and 25 healthy donors was evaluated by flow cytometry, ELISA was used to test the level of TGF-ß1 in blood serum, and analysed the correlation between levels of CD4+ CD25+ regulatory T cells and TGF-ß1.
RESULTS
ITP patients had a lower proportion of CD4+ CD25+ regulatory T cells than the healthy donors (P<0.05). The level of TGF-ß1 in ITP patients was also lower than healthy donors (P<0.05). There was not positive correlation between levels of CD4+ CD25+ regulatory T cells and TGF-ß1 (P<0.05).
CONCLUSION
The results indicate that the decreasing of CD4+ CD25+ regulatory T cells in ITP patients may be connected with cellular immunity disturbance of idiopathic thrombocytopenic purpura. Further research need to be performed in metabolic changes on CD4+ CD25+ regulatory T cells and TGF-ß1 in patients with idiopathic thrombocytopenic purpura.
目的
研究特发性血小板减少性紫癜（ITP）患者外周血中CD4+CD25+调节性T细胞和转化生长因子-β1（TGF-β1）的表达率，以及它们在ITP发病机制中的作用。
方法
采用流式细胞术评估31例患者和25例健康供者外周血中CD4+CD25+调节性T细胞的数量，用酶联免疫吸附测定法（ELISA）检测血清中TGF-β1的水平，并分析CD4+CD25+调节性T细胞水平与TGF-β1水平之间的相关性。
结果
ITP患者CD4+CD25+调节性T细胞的比例低于健康供者（P<0.05）。ITP患者TGF-β1的水平也低于健康供者（P<0.05）。CD4+CD25+调节性T细胞水平与TGF-β1水平之间无正相关（P<0.05）。
结论
结果表明，ITP患者CD4+CD25+调节性T细胞减少可能与特发性血小板减少性紫癜的细胞免疫紊乱有关。需要对特发性血小板减少性紫癜患者CD4+CD25+调节性T细胞和TGF-β1的代谢变化进行进一步研究。
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