果蝇细胞提取物含有一种类转化生长因子-β活性。
Drosophila cell extracts contain a TGF-beta-like activity.
作者信息
Benzakour O, Echalier G, Lawrence D A
机构信息
Institut Curie, UA 532 du CNRS, Centre Universitaire, Orsay, France.
出版信息
Biochem Biophys Res Commun. 1990 Jun 29;169(3):1178-84. doi: 10.1016/0006-291x(90)92020-z.
Extracts of the Drosophila cell line KCo contain a TGF-beta-like activity. This bioactivity, like mammalian TGF-beta, induces the anchorage-independent growth of rat NRK-49F cells in the presence of EGF, inhibits 3H-thymidine incorporation in the mink epithelial cell line CCL-64 and is acid and heat stable but destroyed by dithiothreitol. On a reverse phase column a single bioactive peak eluted at 37-40% acetonitrile. This TGF-beta-like activity in KCo cells inhibits the growth of the homotypic Drosophila cell line 14-11-23, whereas porcine TGF-beta 1 had no effect.
果蝇细胞系KCo的提取物含有一种类转化生长因子-β（TGF-β）活性。这种生物活性，与哺乳动物的TGF-β一样，在表皮生长因子（EGF）存在的情况下可诱导大鼠NRK-49F细胞的非贴壁依赖性生长，抑制水貂上皮细胞系CCL-64中3H-胸腺嘧啶核苷的掺入，并且对酸和热稳定，但会被二硫苏糖醇破坏。在反相柱上，一个单一的生物活性峰在37 - 40%的乙腈浓度下洗脱。KCo细胞中的这种类TGF-β活性可抑制同型果蝇细胞系14 - 11 - 23的生长，而猪TGF-β1则没有这种作用。
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