在花生四烯酸存在的情况下，表皮生长因子对人成纤维细胞的生长抑制作用。

Growth inhibition of human fibroblasts by epidermal growth factor in the presence of arachidonic acid.

作者信息

Hori T, Yamanaka Y, Hayakawa M, Shibamoto S, Oku N, Ito F

机构信息

Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Jun 29;169(3):959-65. doi: 10.1016/0006-291x(90)91987-4.

DOI:10.1016/0006-291x(90)91987-4

PMID:2114113

Abstract

The effect of epidermal growth factor on growth of human fibroblasts was investigated in serum-free medium supplemented with various fatty acids. When linoleic acid, arachidonic acid, or eicosapentaenoic acid was added, each inhibited epidermal growth factor-induced cell growth and showed cytotoxicity at high concentrations (greater than 10 microM). This cytotoxic effect was not observed in the presence of indomethacin, suggesting that prostaglandin production is important in mediation of the growth inhibition. Prostaglandin E2 was increased more than ten thousand times by epidermal growth factor in combination with arachidonic acid.

摘要

在添加了各种脂肪酸的无血清培养基中研究了表皮生长因子对人成纤维细胞生长的影响。当添加亚油酸、花生四烯酸或二十碳五烯酸时，每种脂肪酸均抑制表皮生长因子诱导的细胞生长，并在高浓度（大于10微摩尔）时表现出细胞毒性。在吲哚美辛存在的情况下未观察到这种细胞毒性作用，这表明前列腺素的产生在生长抑制的介导中很重要。表皮生长因子与花生四烯酸联合使用可使前列腺素E2增加一万多倍。