Jiang Huadong, He Xiaohong, Zhang Lixia, Tao Yong, Wang Xiaomei, Gao Ping, Li Daping
College of Life Science, Sichuan University, Chengdu 610064, China.
Wei Sheng Wu Xue Bao. 2010 Oct;50(10):1347-52.
We studied the reduction of soluble and highly toxic selenite to elemental red selenium, under aerobic condition by Pseudomonas alcaliphila MBR, with organic carbon as electron donor.
The strain could grow under pH 6-11 and resist to high concentration of selenite with the minimal inhibitory concentration of 50 mmol/L. After 5 days, the strain used sodium citrate as electron donor, and reduced 2.0 mmol/L selenite to elemental red selenium from the culture fluid, the elemental red selenium was stored outside the cells. The glutathione and nitrate could increase the number of reduction rate.
This study implies the application of Pseudomonas alcaliphila MBR to convert selenite to elemental red selenium.
我们研究了嗜碱假单胞菌MBR在有氧条件下，以有机碳作为电子供体，将可溶性且剧毒的亚硒酸盐还原为元素态红色硒的过程。
该菌株能在pH 6 - 11的条件下生长，并能耐受高浓度亚硒酸盐，其最小抑菌浓度为50 mmol/L。5天后，该菌株以柠檬酸钠作为电子供体，将培养液中2.0 mmol/L的亚硒酸盐还原为元素态红色硒，元素态红色硒储存在细胞外。谷胱甘肽和硝酸盐可提高还原率。
本研究表明嗜碱假单胞菌MBR在将亚硒酸盐转化为元素态红色硒方面具有应用潜力。
