Staicu L C, Ackerson C J, Cornelis P, Ye L, Berendsen R L, Hunter W J, Noblitt S D, Henry C S, Cappa J J, Montenieri R L, Wong A O, Musilova L, Sura-de Jong M, van Hullebusch E D, Lens P N L, Reynolds R J B, Pilon-Smits E A H
Biology Department, Colorado State University, Fort Collins, CO, USA.
UNESCO-IHE Institute for Water Education, Delft, The Netherlands.
J Appl Microbiol. 2015 Aug;119(2):400-10. doi: 10.1111/jam.12842. Epub 2015 Jun 24.
To identify bacteria with high selenium tolerance and reduction capacity for bioremediation of wastewater and nanoselenium particle production.
A bacterial endophyte was isolated from the selenium hyperaccumulator Stanleya pinnata (Brassicaceae) growing on seleniferous soils in Colorado, USA. Based on fatty acid methyl ester analysis and multi-locus sequence analysis (MLSA) using 16S rRNA, gyrB, rpoB and rpoD genes, the isolate was identified as a subspecies of Pseudomonas moraviensis (97.3% nucleotide identity) and named P. moraviensis stanleyae. The isolate exhibited extreme tolerance to SeO3(2-) (up to 120 mmol l(-1)) and SeO4(2-) (>150 mmol l(-1)). Selenium oxyanion removal from growth medium was measured by microchip capillary electrophoresis (detection limit 95 nmol l(-1) for SeO3(2-) and 13 nmol l(-1) for SeO4(2-)). Within 48 h, P. moraviensis stanleyae aerobically reduced SeO3(2-) to red Se(0) from 10 mmol l(-1) to below the detection limit (removal rate 0.27 mmol h(-1) at 30 °C); anaerobic SeO3(2-) removal was slower. No SeO4(2-) removal was observed. Pseudomonas moraviensis stanleyae stimulated the growth of crop species Brassica juncea by 70% with no significant effect on Se accumulation.
Pseudomonas moraviensis stanleyae can tolerate extreme levels of selenate and selenite and can deplete high levels of selenite under aerobic and anaerobic conditions.
Pseudomonas moraviensis subsp. stanleyae may be useful for stimulating plant growth and for the treatment of Se-laden wastewater.
鉴定具有高硒耐受性和还原能力的细菌，用于废水的生物修复和纳米硒颗粒的生产。
从生长在美国科罗拉多州富硒土壤中的十字花科硒超富集植物斯坦利羽扇豆（Stanleya pinnata）中分离出一种细菌内生菌。基于脂肪酸甲酯分析和使用16S rRNA、gyrB、rpoB和rpoD基因的多位点序列分析（MLSA），该分离株被鉴定为摩拉维亚假单胞菌（Pseudomonas moraviensis）的一个亚种（核苷酸同一性为97.3%），并命名为斯坦利摩拉维亚假单胞菌（P. moraviensis stanleyae）。该分离株对亚硒酸根（SeO3(2-)，高达120 mmol l(-1)）和硒酸根（SeO4(2-)，>150 mmol l(-1)）表现出极强的耐受性。通过微芯片毛细管电泳测量生长培养基中硒氧阴离子的去除情况（亚硒酸根的检测限为95 nmol l(-1)，硒酸根的检测限为13 nmol l(-1)）。在48小时内，斯坦利摩拉维亚假单胞菌在有氧条件下将10 mmol l(-1)的亚硒酸根（SeO3(2-)）有氧还原为红色的零价硒（Se(0)），使其降至检测限以下（30°C时去除率为0.27 mmol h(-1)）；厌氧条件下亚硒酸根的去除速度较慢。未观察到硒酸根（SeO4(2-)）的去除。斯坦利摩拉维亚假单胞菌使作物印度芥菜（Brassica juncea）的生长增加了70%，对硒积累没有显著影响。
斯坦利摩拉维亚假单胞菌能够耐受极高水平的硒酸盐和亚硒酸盐，并且在有氧和厌氧条件下都能消耗高水平的亚硒酸盐。
斯坦利摩拉维亚假单胞菌亚种可能有助于促进植物生长和处理含硒废水。
