Anal Chem. 2011 Feb 1;83(3):666-70. doi: 10.1021/ac102666n. Epub 2010 Dec 10.
We report the first next generation sequencing (NGS) application to identify and quantify proteins. Customization of protein specific aptamers enabled direct conversion of serum protein information into NGS read outs. The intrinsic ability of aptamer sequencing to highly multiplex protein detection and quantification, together with the prospect of DNA sequencing further evolving into a commodity technology, could constitute the core of a novel, universal diagnostics paradigm.
我们报告了第一个用于鉴定和定量蛋白质的下一代测序（NGS）应用。定制蛋白质特异性适体使得能够将血清蛋白质信息直接转换为 NGS 读值。适体测序高度多重化蛋白质检测和定量的固有能力，以及 DNA 测序进一步演变成商品技术的前景，可能构成一种新型通用诊断范例的核心。
