Hayashi S, Kubota Y, Shimada S, Hori Y
Dermatology Branch, Yamanashi Medical College, Japan.
Clin Exp Dermatol. 1990 May;15(3):217-21. doi: 10.1111/j.1365-2230.1990.tb02075.x.
We have examined the cytoplasmic distribution of actin and fibronectin in cultured neurofibroma cells (NF cells) derived from a patient with von Recklinghausen's disease by using phase contrast and indirect immunofluorescence microscopy. NF cells were larger in size and more dendritic in shape compared to normal human dermal fibroblasts. NF cells also showed abundant granular staining of actin and a decrease in the linear staining pattern of fibronectin. Furthermore, employing a colony-formation assay on the top of an agar-gel in the presence of fibroblast growth factor (FGF), normal fibroblasts showed a significant number of colonies, whereas NF cells did not demonstrate colony formation even after addition of FGF. These findings suggests that NF cells from patients with von Recklinghausen's disease may have different characteristics when compared with normal fibroblasts, and that NF cells are similar to transformed cells with regard to their actin and fibronectin distribution.
我们通过相差显微镜和间接免疫荧光显微镜检查了一名患有冯·雷克林豪森氏病患者的培养神经纤维瘤细胞（NF细胞）中肌动蛋白和纤连蛋白的细胞质分布。与正常人皮肤成纤维细胞相比，NF细胞体积更大，形状更具树突状。NF细胞还显示出丰富的肌动蛋白颗粒染色以及纤连蛋白线性染色模式的减少。此外，在成纤维细胞生长因子（FGF）存在的情况下，在琼脂凝胶顶部进行集落形成试验，正常成纤维细胞显示出大量集落，而即使添加FGF后，NF细胞也未显示集落形成。这些发现表明，与正常成纤维细胞相比，冯·雷克林豪森氏病患者的NF细胞可能具有不同的特征，并且NF细胞在肌动蛋白和纤连蛋白分布方面与转化细胞相似。
