Sasaki T, Arai K, Nagai Y
Department of Dermatology, Yokohama City University School of Medicine, Japan.
J Dermatol. 1992 Oct;19(10):598-601. doi: 10.1111/j.1346-8138.1992.tb03736.x.
Cells from cutaneous neurofibromas of three patients with von Recklinghausen's disease and skin fibroblasts from four healthy adults were cultured. After two passages, DNA and collagen syntheses were determined by measuring incorporation of [3H] thymidine and [3H] proline respectively, and expressed as the values per unit DNA content. These values from neurofibroma cells were increased by 54% in DNA synthesis and 60% (p < 0.05) in nondialyzable hydroxyproline synthesis, suggesting that neurofibroma cells in culture even after several passages still possess those characteristics corresponding to their pathological features in vivo. Collagen synthesized by neurofibroma cells appears to be normal as judged by intracellular degradation rates and SDS-polyacrylamide gel electrophoresis.
培养了三名冯雷克林霍增氏病患者皮肤神经纤维瘤的细胞以及四名健康成年人的皮肤成纤维细胞。传代两次后，分别通过测量[3H]胸腺嘧啶核苷和[3H]脯氨酸的掺入量来测定DNA和胶原蛋白的合成，并以每单位DNA含量的值表示。神经纤维瘤细胞的这些值在DNA合成中增加了54%，在不可透析羟脯氨酸合成中增加了60%（p<0.05），这表明培养的神经纤维瘤细胞即使经过几次传代后仍具有与其体内病理特征相对应的那些特性。根据细胞内降解率和SDS-聚丙烯酰胺凝胶电泳判断，神经纤维瘤细胞合成的胶原蛋白似乎是正常的。
