Albino-Teixeira A, Azevedo I, Branco D, Osswald W
Laboratório de Farmacologia, Faculdade de Medicina do Porto, Portugal.
Eur J Pharmacol. 1990 Apr 10;179(1-2):141-9. doi: 10.1016/0014-2999(90)90411-x.
Surgical denervation of the lateral saphenous vein of the dog causes marked extraneuronal changes, both of a morphological and functional type. In an attempt to investigate the factor(s) responsible for the trophic effects exerted by the sympathetic innervation on the dog saphenous vein we studied the effects of noradrenaline, adenosine, inosine and N-ethylcarboxamidoadenosine (NECA) on vascular tissue after sympathetic denervation. The saphenous vein was denervated using either surgical or chemical (6-hydroxydopamine, 6-OHDA) methods. Noradrenaline (0.1 microgram/kg per h), adenosine (10 micrograms/kg per h), inosine (10 micrograms/kg per h) or NECA (0.1 microgram/kg per h) were delivered continuously for 5 days through Alzet minipumps connected to the vein. 6-OHDA-induced denervation resulted in morphological changes similar to those described for surgical denervation. Smooth muscle cells and fibroblasts showed ultrastructural signs of increased synthetic activity and their size was significantly increased. In confirmation of earlier studies, constant i.v. infusions of noradrenaline did not prevent the morphological changes induced by denervation. Adenosine prevented the morphological changes induced by chemical or surgical denervation. Similarly to adenosine, infused NECA prevented the structural consequences of denervation. In contrast, inosine did not prevent the changes caused by surgical denervation. The results are compatible with an involvement of purines in the trophic effects of sympathetic innervation. Moreover, the effects of adenosine do not appear to be mediated by inosine.
对犬隐静脉进行手术去神经支配会导致明显的神经外变化，包括形态学和功能类型的变化。为了研究交感神经支配对犬隐静脉产生营养作用的相关因素，我们研究了去甲肾上腺素、腺苷、肌苷和N - 乙基羧酰胺腺苷（NECA）对去神经支配后血管组织的影响。采用手术或化学方法（6 - 羟基多巴胺，6 - OHDA）对隐静脉进行去神经支配。通过连接到静脉的Alzet微型泵连续5天给予去甲肾上腺素（0.1微克/千克每小时）、腺苷（10微克/千克每小时）、肌苷（10微克/千克每小时）或NECA（0.1微克/千克每小时）。6 - OHDA诱导的去神经支配导致的形态学变化与手术去神经支配所描述的相似。平滑肌细胞和成纤维细胞显示出合成活性增加的超微结构迹象，并且它们的大小显著增加。正如早期研究所证实的，持续静脉输注去甲肾上腺素并不能预防去神经支配诱导的形态学变化。腺苷可预防化学或手术去神经支配诱导的形态学变化。与腺苷类似，输注的NECA可预防去神经支配的结构后果。相比之下，肌苷不能预防手术去神经支配引起的变化。这些结果与嘌呤参与交感神经支配的营养作用相一致。此外，腺苷的作用似乎不是由肌苷介导的。
