Sarmento A, Soares-da-Silva P, Teixeira A A, Azevedo I
Laboratório de Farmacologia, Faculdade de Medicina, Porto, Portugal.
J Auton Pharmacol. 1987 Jun;7(2):119-26. doi: 10.1111/j.1474-8673.1987.tb00141.x.
1 Denervation was induced in dogs by 6-hydroxydopamine (6-OHDA) treatment. The effects of this on cell nucleus activity of smooth muscle cells and fibroblasts of the mesenteric artery (main trunk and jejunal branches) and of myocardial cells, fibroblasts and endothelial capillary cells of the heart were studied. 2 Changes in the nucleii or cell size measured with light microscopy morphometric techniques and frequency of nucleoli in myocardial cells were chosen as indices of cell nucleus activity state. 3 Noradrenaline depletion 5 days after initiation of 6-OHDA administration was much more marked in the heart (with a content of 10% of control values) than in the arterial vessels (with a content of 50% of control values). 4 Denervation by 6-OHDA led to an increase in the synthetic activity state of the cell nucleus in all cell types and tissues. 5 Plasma noradrenaline and adrenaline levels were strikingly increased by 6-OHDA treatment. 6 The results strongly support the hypothesis of a nuclear modulating effect of the sympathetic innervation upon the effector cells. The incapacity of high noradrenaline plasma levels to prevent the consequences of sympathetic denervation on the effector cells appears to indicate that in the sympathetic innervation there is a 'trophic factor' which is not identical with noradrenaline.
1 通过6-羟基多巴胺（6-OHDA）处理在犬中诱导去神经支配。研究了其对肠系膜动脉（主干和空肠分支）的平滑肌细胞和成纤维细胞以及心脏的心肌细胞、成纤维细胞和内皮毛细血管细胞核活性的影响。2 用光镜形态计量技术测量的细胞核或细胞大小的变化以及心肌细胞中核仁的频率被选作细胞核活性状态的指标。3 在开始给予6-OHDA 5天后，心脏中的去甲肾上腺素耗竭（含量为对照值的10%）比动脉血管中（含量为对照值的50%）更为明显。4 6-OHDA诱导的去神经支配导致所有细胞类型和组织中细胞核的合成活性状态增加。5 6-OHDA处理使血浆去甲肾上腺素和肾上腺素水平显著升高。6 结果有力地支持了交感神经支配对效应细胞具有核调节作用的假说。高血浆去甲肾上腺素水平无法预防交感神经去支配对效应细胞的影响，这似乎表明在交感神经支配中存在一种与去甲肾上腺素不同的“营养因子”。
