Laboratory of Biochemistry-Toxicology, University Hospital of Monastir, Monastir, Tunisia.
Clin Chem Lab Med. 2011 Mar;49(3):479-83. doi: 10.1515/CCLM.2011.062. Epub 2010 Dec 14.
Cigarette smoking has been recognized as a major risk factor for cardiovascular disease, while the role of homocysteine is still not clear. This study investigated the effects of smoking on plasma homocysteine concentration and determined the correlation between this parameter and biological markers of tobacco use, such as plasma thiocyanate and urine cotinine.
Folate, vitamin B12 and homocysteine were measured in 300 subjects: 138 non-smokers and 162 smokers using immunoassay methods. Cotinine was measured using an enzymatic colorimetric method and thiocyanate by a selective electrode.
In smokers, we found a significant increase in homocysteine and a decrease in folate and vitamin B12 levels compared to non-smokers. Homocysteine was strongly correlated with the duration of use and the number of cigarettes consumed. Folate and vitamin B12 were significantly reduced in subjects smoking for more than 20 years compared to those who smoked less than 5 years. Among smokers, we noted a positive correlation between homocysteine and both plasma thiocyanates and cotininuria, and a negative-correlation between cotininuria and plasma folate.
Cigarette smoking increases homocysteine, which is strongly correlated with cotininuria and plasma thiocyanates. Moreover, smokers had tendency to develop hypofolatemia and hypovitamin B12, particularly when the duration of consumption exceeded 20 years.
吸烟已被认为是心血管疾病的主要危险因素，而同型半胱氨酸的作用尚不清楚。本研究调查了吸烟对血浆同型半胱氨酸浓度的影响，并确定了该参数与烟草使用的生物标志物（如血浆硫氰酸盐和尿可替宁）之间的相关性。
采用免疫测定法测量 300 例受试者（非吸烟者 138 例，吸烟者 162 例）的叶酸、维生素 B12 和同型半胱氨酸。采用酶比色法测定可替宁，采用选择性电极法测定硫氰酸盐。
与非吸烟者相比，吸烟者的同型半胱氨酸显著升高，叶酸和维生素 B12 水平降低。同型半胱氨酸与使用时间和吸烟数量呈强相关。与吸烟 5 年以下的人相比，吸烟 20 年以上的人叶酸和维生素 B12 明显降低。在吸烟者中，我们注意到同型半胱氨酸与血浆硫氰酸盐和尿可替宁之间呈正相关，而尿可替宁与血浆叶酸之间呈负相关。
吸烟增加同型半胱氨酸，其与尿可替宁和血浆硫氰酸盐密切相关。此外，吸烟者有发生低叶酸血症和维生素 B12 缺乏的趋势，尤其是当吸烟时间超过 20 年时。
