Countryside Equine Hospital, Louisville, TN, USA.
J Vet Intern Med. 2011 Jan-Feb;25(1):132-7. doi: 10.1111/j.1939-1676.2010.0645.x. Epub 2010 Dec 8.
Neonatal hypoxic-ischemic encephalopathy (NHIE) is a disease affecting newborn foals for which there is no antemortem diagnostic test.
Ubiquitin C-terminal hydrolase 1 (UCHL1) and the phosphorylated axonal forms of neurofilament H (pNF-H) are markers of brain injury in foals with NHIE.
Thirty-three foals with a clinical diagnosis consistent with NHIE and 17 healthy foals.
Retrospective study. Concentrations of UCHL1 and pNF-H in plasma were measured by ELISA. The performance of the assays for the diagnosis of NHIE was assessed by receiver operating characteristic curve analysis. Concentrations of UCHL1 and pNF-H were measured throughout the brains of 2 healthy foals.
The diagnostic performance of UCHL1 (AUC = 0.86) was significantly higher (P = .001) than that of pNF-H (0.52) for the diagnosis of NHIE. Median concentrations of UCHL1 (6.57 ng/mL; 2.35-11.90 ng/mL) in foals with a clinical diagnosis of NHIE were significantly (P < .001) higher than those of healthy controls (2.52 ng/mL; 1.4-4.01 ng/mL). The right sided reference interval for UCHL1 concentrations in healthy foals was 0-4.01 ng/mL. The sensitivity and specificity of UCHL1 (>4.01 ng/mL) for diagnosis of NHIE were 70% (51-84%) and 94% (72-99%), respectively. UCHL1 concentrations were higher in gray than white matter, while pNF-H concentrations were higher in white than gray matter.
UCHL1 has potential as a marker of brain injury in foals with NHIE.
新生儿缺氧缺血性脑病（NHIE）是一种影响新生驹的疾病，目前尚无生前诊断测试。
泛素 C 端水解酶 1（UCHL1）和磷酸化的神经丝 H（pNF-H）的轴突形式是 NHIE 驹脑损伤的标志物。
33 匹临床诊断符合 NHIE 的驹和 17 匹健康驹。
回顾性研究。通过 ELISA 测量 UCHL1 和 pNF-H 在血浆中的浓度。通过接收者操作特征曲线分析评估这些检测方法对 NHIE 诊断的性能。在 2 匹健康驹的整个大脑中测量 UCHL1 和 pNF-H 的浓度。
UCHL1 的诊断性能（AUC = 0.86）明显高于 pNF-H（0.52）（P =.001），用于 NHIE 的诊断。临床诊断为 NHIE 的驹的 UCHL1 中位数浓度（6.57 ng/mL；2.35-11.90 ng/mL）明显高于健康对照组（2.52 ng/mL；1.4-4.01 ng/mL）（P <.001）。健康驹 UCHL1 浓度的右侧参考区间为 0-4.01 ng/mL。UCHL1（>4.01 ng/mL）诊断 NHIE 的敏感性和特异性分别为 70%（51-84%）和 94%（72-99%）。UCHL1 浓度在灰质中较高，而 pNF-H 浓度在白质中较高。
UCHL1 可能是 NHIE 驹脑损伤的标志物。
