载脂蛋白A-II的比浊法测定

Turbidimetric assay of apolipoprotein A-II.

作者信息

Schriewer H, Emke F, Assmann G

出版信息

J Clin Chem Clin Biochem. 1985 Jun;23(6):355-9. doi: 10.1515/cclm.1985.23.6.355.

DOI:10.1515/cclm.1985.23.6.355

Abstract

A turbidimetric assay for the quantification of apolipoprotein A-II in serum is described. Its adaptation for the Cobas Bio Analyser (Hoffmann La Roche) is reported. Regression analysis of apolipoprotein A-II values measured by turbidimetry revealed a good measure of agreement with the data obtained by radial immunodiffusion (RID) (r = 0.90, y = 0.97x + 0.021, n = 100) as well as with data obtained by immunonephelometry (r = 0.838, y = 0.925x + 0.029, n = 34). A variation coefficient of 3.2% (n = 18) was found in relation to the precision in the series and a variation coefficient of 3.5% (n = 36) in relation to day to day precision.

摘要

本文描述了一种用于定量测定血清中载脂蛋白A-II的比浊法。报告了其在Cobas Bio分析仪（霍夫曼·罗氏公司）上的应用。通过比浊法测定的载脂蛋白A-II值的回归分析表明，与通过放射免疫扩散法（RID）获得的数据（r = 0.90，y = 0.97x + 0.021，n = 100）以及与通过免疫比浊法获得的数据（r = 0.838，y = 0.925x + 0.029，n = 34）具有良好的一致性。相对于系列中的精密度，发现变异系数为3.2%（n = 18），相对于每日精密度，变异系数为3.5%（n = 36）。