Faculty of Forestry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Plant Biol (Stuttg). 2011 Jan;13(1):194-200. doi: 10.1111/j.1438-8677.2010.00326.x.
To examine variation and taxonomic recognition of Pinus nigra (European black pine) at the intraspecific level, chromosomal distribution of 5S and 18S-5.8S-26S rDNA loci revealed by fluorescent in situ hybridisation (FISH) and fluorochrome banding with chromomycin A(3) and DAPI were analysed among allopatric populations belonging to different subspecies. Despite prevalent opinion on predominantly conserved and homogenous conifer karyotypes, several patterns were observed. Surprisingly, interstitial 18S rDNA loci and DAPI heterochromatin staining after FISH showed variations in distribution and localisation. Three subspecies shared a pattern with nine 18S rDNA loci (ssp. nigra, pallasiana and laricio) while ssp. dalmatica and salzmannii had eight rDNA loci. DAPI banding displayed two patterns, one with a high number of signals (ssp. nigra, pallasiana and dalmatica) and the other with a lower number of signals (ssp. salzmannii and laricio). We conclude that our results cannot provide proof for either classification scheme for the P. nigra complex, but rather demonstrate the variability of different heterochromatin fractions at the intraspecific level.
为了研究欧洲黑松（Pinus nigra）种内水平的变异和分类识别，通过荧光原位杂交（FISH）和用 Chromomycin A（3）和 DAPI 进行荧光带型分析，研究了属于不同亚种的远缘种群中 5S 和 18S-5.8S-26S rDNA 基因座的染色体分布。尽管人们普遍认为针叶树的核型主要是保守和均匀的，但还是观察到了几种模式。令人惊讶的是，FISH 后 18S rDNA 基因座的间位和 DAPI 异染色质染色显示出分布和定位的变化。三个亚种共享九个 18S rDNA 基因座的模式（ssp. nigra、pallasiana 和 laricio），而 ssp. dalmatica 和 salzmannii 有八个 rDNA 基因座。DAPI 带型显示出两种模式，一种有大量信号（ssp. nigra、pallasiana 和 dalmatica），另一种有较少的信号（ssp. salzmannii 和 laricio）。我们的结论是，我们的结果不能为 P. nigra 复合体的任何分类方案提供证明，而只是证明了不同异染色质分数在种内水平上的可变性。
