Clinical Microbiology Laboratory, Aga Khan University Hospital, Karachi, Pakistan.
Int J Tuberc Lung Dis. 2010 Dec;14(12):1644-6.
We report the efficiency and cost-effectiveness of p-nitrobenzoic acid (PNB) testing in Middlebrook 7H10 agar medium for the identification of Mycobacterium tuberculosis complex (MTC). PNB-7H10 was compared with PNB-MGIT and BACTEC-NAP using 200 clinical mycobacterial isolates. PNB-7H10 showed 100% agreement with PNB-MGIT and BACTEC-NAP tests, and reduced the cost of PNB-MGIT test by 80%. PNB-7H10 agar is therefore an effective alternative to the costly PNB-MGIT and BACTEC-NAP tests, especially in resource-poor settings.
我们报告了在 Middlebrook 7H10 琼脂培养基中使用对硝基苯甲酸(PNB)检测鉴定结核分枝杆菌复合群(MTC)的效率和成本效益。将 PNB-7H10 与 PNB-MGIT 和 BACTEC-NAP 进行了比较,共使用了 200 株临床分枝杆菌分离株。PNB-7H10 与 PNB-MGIT 和 BACTEC-NAP 检测结果完全一致,并将 PNB-MGIT 检测成本降低了 80%。因此,PNB-7H10 琼脂是昂贵的 PNB-MGIT 和 BACTEC-NAP 检测的有效替代品,尤其是在资源匮乏的环境中。