Agri-Foods & Health Section, AgResearch, Ruakura Research Centre, PB 3123, Hamilton, New Zealand.
J Chromatogr A. 2011 Jan 14;1218(2):316-23. doi: 10.1016/j.chroma.2010.11.011. Epub 2010 Nov 16.
The fatty acid composition of milk is of considerable interest due to their nutritional and functional properties. Although rapid milk fat separation and transesterification procedures have been developed, the overall procedure remains time consuming, specially, for the analysis of a large number of samples. In this work, a fast and simple method for direct profiling of fatty acids from milk using thermochemolysis has been developed. This method has the capability of directly analyse fatty acids from one drop of milk without fat extraction or cleanup. Our approach for thermochemolysis is based on thermal desorption integrated with a cold trap inlet. The optimized method does not present isomerisation/degradation of polyunsaturated fatty acid and shows milk fatty acid profiles comparable to the conventional method based on fat extraction and alkaline transesterification. Overall, this method has demonstrated significant potential for high throughput analysis of fatty acids in milk.
由于其营养和功能特性，牛奶中的脂肪酸组成引起了相当大的关注。尽管已经开发出了快速的牛奶脂肪分离和酯交换程序，但总体程序仍然很耗时，特别是对于大量样品的分析。在这项工作中，开发了一种使用热化学分解法直接分析牛奶中脂肪酸的快速简单方法。该方法无需脂肪提取或净化，即可直接分析一滴牛奶中的脂肪酸。我们的热化学分解方法基于热解吸与冷阱入口相结合。优化后的方法不会导致多不饱和脂肪酸的异构化/降解，并显示出与基于脂肪提取和碱性酯交换的常规方法相当的牛奶脂肪酸图谱。总的来说，该方法在牛奶中脂肪酸的高通量分析方面具有很大的潜力。
