Equipe de Virologie, UMR GDPP, INRA, Université Bordeaux 2, IBVM, BP81, 33883 Villenave d'Ornon Cedex, France.
Virus Res. 2011 Feb;155(2):440-5. doi: 10.1016/j.virusres.2010.11.013. Epub 2010 Dec 7.
The complete genomic sequences of four Prunus-infecting Apricot latent virus (ApLV) like isolates were determined and used to analyze the taxonomic position and variability of these viruses. The results indicate that all isolates show a typical Foveavirus genetic organization. Despite an average 23% nucleotide divergence, they show strong colinearity with only three regions of significant indel variability, in the internal and 3' non-coding regions and variable N-terminal half of the coat protein (CP). Sequence comparisons using the polymerase (Pol) and CP genes provide a conflicting taxonomic picture, with divergence level in the Pol and CP genes suggesting the existence of a single or of two species, respectively. However, a range of considerations argue that all four isolates should likely be considered as belonging to the ApLV species. ApLV is closely related to Apple stem pitting virus and could be considered a sister species to it, with ASPV being specialized to infect members of the Maloideae family and ApLV members of the Prunoideae. Analysis of selection pressures affecting the five open reading frames of ApLV and ASPV identified two regions under strong purifying selection, that coding for the conserved C-terminal half of the CP and the gene coding for the first protein of the triple gene block (TGBp1).
四个李属感染李潜隐病毒（ApLV）样分离物的完整基因组序列已被确定，并用于分析这些病毒的分类地位和变异性。结果表明，所有分离物均表现出典型的 Foveavirus 遗传组织。尽管核苷酸差异平均为 23%，但它们与内部和 3'非编码区以及衣壳蛋白（CP）可变 N 端的三个具有显著插入缺失变异性的区域具有很强的共线性。使用聚合酶（Pol）和 CP 基因进行的序列比较提供了一个相互矛盾的分类图景，Pol 和 CP 基因的分化水平表明存在一个或两个种。然而，一系列考虑因素表明，所有四个分离物都可能被认为属于 ApLV 种。ApLV 与苹果茎痘病毒密切相关，可被视为其姊妹种，ASPV 专门感染桃金娘科成员，而 ApLV 则感染李属成员。对影响 ApLV 和 ASPV 五个开放阅读框的选择压力的分析确定了两个受到强烈净化选择的区域，即编码 CP 保守 C 端半部分和三基因块（TGBp1）第一个蛋白的基因。
