Gao J, Liu J, Fan D, Xu H, Xiong Y, Wang Y, Xu W, Wang Y, Cheng Y, Zheng G
Department of Digestive Diseases, Wuhan General Hospital of Guangzhou Command PLA, Wuhan, Hubei Province, PR China.
Pathol Biol (Paris). 2011 Dec;59(6):298-302. doi: 10.1016/j.patbio.2010.11.001. Epub 2010 Dec 8.
Deregulated expression of Notch molecules is observed in many malignant tumors, however, the expression of Notch1 and Jagged1 in colon adenocarcinoma is still unknown. This study is to investigate the expression of Notch1 and Jagged1 in human colon adenocarcinoma. Sixty-five human colon adenocarcinoma and 60 adjacent nontumor colon tissue sections were detected by immunohistochemistry. Ten paired fresh surgical human colon adenocarcinoma and adjacent nontumor colon samples were analyzed by Western blot and RT-PCR. Both Notch1 and Jagged1 were expressed in the cytoplasm of neoplastic cells of colon adenocarcinoma tissue. The protein and mRNA levels of both molecules were higher in colon adenocarcinoma than in adjacent nontumor tissue. Moreover, Notch1 was positively correlated with tumor stage. This investigation demonstrates that Notch1 and Jagged1 are up-regulated in human colon adenocarcinoma and suggests that Notch1/Jagged1 signaling might play a role in the development of colon adenocarcinoma.
在许多恶性肿瘤中都观察到Notch分子的表达失调，然而，Notch1和Jagged1在结肠腺癌中的表达情况仍不清楚。本研究旨在探讨Notch1和Jagged1在人结肠腺癌中的表达。采用免疫组织化学方法检测65例人结肠腺癌和60例相邻非肿瘤结肠组织切片。采用蛋白质印迹法和逆转录-聚合酶链反应（RT-PCR）分析10对新鲜手术切除的人结肠腺癌及相邻非肿瘤结肠样本。Notch1和Jagged1均在结肠腺癌组织的肿瘤细胞胞质中表达。这两种分子的蛋白质和mRNA水平在结肠腺癌中均高于相邻非肿瘤组织。此外，Notch1与肿瘤分期呈正相关。本研究表明，Notch1和Jagged1在人结肠腺癌中上调，提示Notch1/Jagged1信号通路可能在结肠腺癌的发生发展中起作用。
