Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences-SLU, Uppsala, Sweden.
Anim Reprod Sci. 2011 Jan;123(1-2):64-9. doi: 10.1016/j.anireprosci.2010.11.005. Epub 2010 Nov 12.
There is considerable interest world-wide in reducing the use of antibiotics to stem the development of antibiotic-resistant strains of bacteria. An alternative to the routine addition of antibiotics to semen extenders in livestock breeding would be to separate the spermatozoa from bacterial contaminants in the semen immediately after collection. The present study was designed to determine whether such separation was possible by Single Layer Centrifugation (SLC) using the colloid Androcoll™-P. The results showed that complete removal (6 out of 10 samples), or considerable reduction of bacterial contaminants (4 out of 10 samples) was possible with this method. The type of bacteria and/or the length of time between collection and SLC-processing affected the removal of bacteria, with motile flagellated bacteria being more likely to be present after SLC than non-flagellated bacteria. Although further studies are necessary, these preliminary results suggest that the use of SLC when processing boar semen for AI doses might enable antibiotic usage in semen extenders to be reduced.
全世界都非常关注减少抗生素的使用，以阻止抗药性细菌的产生。在畜牧业中，代替在精液稀释液中常规添加抗生素的方法是，在收集精液后立即将精子与精液中的细菌污染物分离。本研究旨在确定使用胶体 Androcoll™-P 通过单层离心（SLC）是否可以实现这种分离。结果表明，用这种方法可以完全去除（10 个样本中有 6 个）或大大减少细菌污染物（10 个样本中有 4 个）。细菌的类型和/或收集与 SLC 处理之间的时间长短会影响细菌的去除，运动的有鞭毛的细菌比没有鞭毛的细菌更有可能在 SLC 后存在。尽管还需要进一步的研究，但这些初步结果表明，在进行人工授精剂量的种猪精液处理时使用 SLC，可能会减少抗生素在精液稀释液中的使用。
