Biophysiologie cellulaires, faculté des sciences de Bizerte, 7021 Zarzouna, Tunisia.
C R Biol. 2010 Nov-Dec;333(11-12):801-7. doi: 10.1016/j.crvi.2010.09.002. Epub 2010 Oct 13.
Pea seeds (Pisum sativum L.) were germinated by soaking in distilled water or 5mM CdCl2 for 5 days. The relationships among Cd treatment, germination rate, embryonic axis growth, NAD(P)H levels and NAD(P)H oxidase activities in mitochondrial and peroxisomal fractions of cotyledons and embryonic axis were investigated. Heavy metal stress provoked a diminution in germination percent and embryonic axis growth, as compared to the control. A drastic disorder in reducing power was imposed after exposure to cadmium. Heavy metal caused a significant increase in the redox ratio of coenzymes. NADPH oxidase is considered to be oxidative stress-related enzymes. The NAD(P)H oxidase activities were strongly stimulated after Cd exposure. The changes in redox and oxidative properties are discussed in relation to the delay in seed germination and embryonic axis growth.
豌豆种子（Pisum sativum L.）在蒸馏水中或 5mM CdCl2 中浸泡 5 天进行发芽。研究了 Cd 处理、发芽率、胚轴生长、NAD(P)H 水平以及子叶和胚轴线粒体和过氧化物酶体部分 NAD(P)H 氧化酶活性之间的关系。与对照相比，重金属胁迫会降低发芽率和胚轴生长。暴露于镉后，还原力严重失调。重金属导致辅酶的氧化还原比显著增加。NADPH 氧化酶被认为是与氧化应激相关的酶。暴露于 Cd 后，NAD(P)H 氧化酶活性被强烈刺激。讨论了氧化还原和氧化性质的变化与种子发芽和胚轴生长延迟的关系。
