Wurche T, Wagner S, Mauch H
Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Berlin.
Pneumologie. 1990 Feb;44 Suppl 1:455-7.
M. avium-complex (MAC) is the cause of the most bacterial infections in AIDS patients. Because of the high resistance of MAC, a rapid differentiation between M. tuberculosis and MAC is of great interest. In an enzyme-linked immunosorbent assay (ELISA) we tested three monoclonal antibodies BS 103, BS 104, BS 113 and the combination of BS 103/BS 113, which bind selectively to the cell wall of M. tuberculosis. 98 MAC isolates from AIDS patients and 233 M. tuberculosis isolates from patients with lung disease were tested after primary culture of the bacteria on Lowenstein-Jensen media. ELISA could clearly discriminate MAC from M. tuberculosis with a specificity of 100% and a sensitivity of 98-100%. With these monoclonal antibodies the time for differentiation can be reduced by several weeks.
鸟分枝杆菌复合群（MAC）是艾滋病患者中大多数细菌感染的病因。由于MAC具有高度耐药性，快速区分结核分枝杆菌和MAC具有重要意义。在酶联免疫吸附测定（ELISA）中，我们测试了三种单克隆抗体BS 103、BS 104、BS 113以及BS 103/BS 113的组合，它们能选择性地结合结核分枝杆菌的细胞壁。在罗-琴培养基上对细菌进行初次培养后，检测了98株来自艾滋病患者的MAC分离株和233株来自肺病患者的结核分枝杆菌分离株。ELISA能够以100%的特异性和98%-100%的敏感性清楚地鉴别MAC和结核分枝杆菌。使用这些单克隆抗体，鉴别时间可缩短数周。
