Krest'anpol M, Kolk A H, Kaustová J
National Institute of Public Health, Prague, Czech Republic.
Cent Eur J Public Health. 1993 Jun;1(1):31-4.
An enzyme-linked immunosorbent assay (ELISA) for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex and Mycobacterium kansasii originally described by R. Schöningh, C. P. J. H. Verstijnen, S. Kuijper and A. H. J. Kolk (1) was used for identification of mycobacteria from three week old primary cultures. A panel of six monoclonal antibodies (MoAbs) was used: two were specific for Mycobacterium tuberculosis (M. tuberculosis) complex, one for M. kansasii, one was directed against M. avium complex and two were broadly reacting with all mycobacterial species. The ELISA was introduced to a microbiology laboratory located in an area where M. kansasii infections are endemic. All acid-fast bacteria isolated from sputum samples over one month period were identified by ELISA and culture. All fifteen M. tuberculosis isolates and all seventeen M. kansasii were correctly identified by ELISA before culture results were known. Two of three M. avium complex strains could be identified in ELISA as belonging to the M. avium complex using the M. avium complex specific monoclonal antibody.
一种最初由R. Schöningh、C. P. J. H. Verstijnen、S. Kuijper和A. H. J. Kolk（1）描述的用于鉴定属于结核分枝杆菌复合群和堪萨斯分枝杆菌的培养分枝杆菌的酶联免疫吸附测定（ELISA），被用于从三周龄的初代培养物中鉴定分枝杆菌。使用了一组六种单克隆抗体（MoAbs）：两种对结核分枝杆菌（M. tuberculosis）复合群具有特异性，一种对堪萨斯分枝杆菌具有特异性，一种针对鸟分枝杆菌复合群，两种与所有分枝杆菌物种广泛反应。该ELISA被引入到一个位于堪萨斯分枝杆菌感染流行地区的微生物实验室。在一个月的时间里，通过ELISA和培养对从痰液样本中分离出的所有抗酸细菌进行了鉴定。在培养结果出来之前，所有15株结核分枝杆菌分离株和所有17株堪萨斯分枝杆菌均通过ELISA被正确鉴定。使用鸟分枝杆菌复合群特异性单克隆抗体，在ELISA中可以将三株鸟分枝杆菌复合群菌株中的两株鉴定为属于鸟分枝杆菌复合群。
