Laboratory of Molecular and Cell Genetics, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
FEBS Lett. 2011 Jan 3;585(1):133-8. doi: 10.1016/j.febslet.2010.12.002. Epub 2010 Dec 10.
Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress. The ER stress sensor inositol requiring enzyme-1beta (IRE1β), which is specifically expressed in intestinal epithelial cells, is thought to be involved in translational repression. However, its mechanism of action is not fully understood. Using a reporter that can evaluate and distinguish between translation efficiency in the cytosol and on the ER membrane, we show here that IRE1β represses translation on the ER membrane but not in the cytosol, and that this selective repression depends on the RNase activity of IRE1β.
内质网（ER）中未折叠蛋白的积累会导致 ER 应激。内质网应激传感器肌醇需求酶 1β（IRE1β）在肠上皮细胞中特异性表达，被认为参与翻译抑制。然而，其作用机制尚不完全清楚。使用一种可以评估和区分细胞质和内质网膜上翻译效率的报告基因，我们在这里表明，IRE1β在内质网膜上而非细胞质中抑制翻译，并且这种选择性抑制依赖于 IRE1β的核糖核酸酶活性。
