University of Santiago de Compostela, Department of Chemical Engineering, Santiago de Compostela, Spain.
Bioresour Technol. 2011 Feb;102(3):2549-54. doi: 10.1016/j.biortech.2010.11.084. Epub 2010 Nov 25.
The physiological and kinetic behavior of a denitrifying granular sludge exposed to different sulfide loading rates (55-295 mg/L d) were evaluated in a UASB reactor fed with acetate, ammonium and nitrate. At any sulfide loading rates, the consumption efficiencies of sulfide, acetate and ammonium were above 95%, while nitrate consumption efficiencies were around 62-72%. At the highest sulfide loading rate the ammonium was used as electron donor for N(2) production. The increase of sulfide loading rate also affected the fate of sulfide oxidation, since elemental sulfur was the main end product instead of sulfate. However, the lithotrophic denitrifying kinetic was not affected. FISH oligonucleotide probes for Thiobacillus denitrificans, Thiomiscropira denitrificans, genus Paracoccus and Pseudomonas spp. were used to follow the microbial ecology. The results of this work have shown that four pollutants could simultaneously be removed, namely, sulfide, ammonium, acetate and nitrate under well defined denitrifying conditions.
在 UASB 反应器中，采用乙酸盐、铵盐和硝酸盐作为底物，评估了在不同硫化物负荷率（55-295 mg/L·d）下，反硝化颗粒污泥的生理和动力学行为。在任何硫化物负荷率下，硫化物、乙酸盐和铵盐的消耗效率均高于 95%，而硝酸盐的消耗效率约为 62-72%。在最高的硫化物负荷率下，铵盐被用作 N(2)生产的电子供体。硫化物负荷率的增加也影响了硫化物氧化的命运，因为单质硫是主要的终产物，而不是硫酸盐。然而，自养反硝化动力学并未受到影响。使用针对脱氮硫杆菌、脱氮硫微菌、副球菌属和假单胞菌属的 FISH 寡核苷酸探针来跟踪微生物生态学。这项工作的结果表明，在明确的反硝化条件下，可以同时去除四种污染物，即硫化物、铵盐、乙酸盐和硝酸盐。
