Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan, Ankara, Turkey.
Talanta. 2011 Jan 15;83(3):780-8. doi: 10.1016/j.talanta.2010.10.049. Epub 2010 Nov 4.
The anticancer drug, leuprolide (LPR) bound to double-stranded fish sperm DNA (dsDNA) which was immobilized onto the surface of an anodically activated pencil graphite electrode (PGE), was employed for designing a sensitive biosensor. The interaction of leuprolide (LPR) with double-stranded DNA (dsDNA) immobilized onto pencil graphite electrode (PGE) have been studied by electrochemical methods. The mechanism of the interaction was investigated and confirmed by differential pulse voltammetry using two different interaction methods; at the PGE surface and in the solution phase. The decrease in the guanine oxidation peak current was used as an indicator for the interaction in acetate buffer at pH 4.80. The response was optimized with respect to accumulation time, potential, drug concentration, and reproducibility for both interaction methods. The linear response was obtained in the range of 0.20-6.00 ppm LPR concentration with a detection limit of 0.06 ppm on DNA modified PGE and between 0.20 and 1.00 ppm concentration range with detection limit of 0.04 ppm for interaction in solution phase method. LPR showed an irreversible oxidation behavior at all investigated pH values on a bare PGE. Differential pulse adsorptive stripping (AdSDPV) voltammetric method was developed for the determination of LPR. Under these conditions, the current showed a linear dependence with concentration within a range of 0.005-0.20 ppm with a detection limit of 0.0014 ppm. Each determination method was fully validated and applied for the analysis of LPR in its pharmaceutical dosage form.
抗癌药物亮丙瑞林（LPR）与固定在阳极活化铅笔石墨电极（PGE）表面的双链鱼精 DNA（dsDNA）结合，用于设计灵敏的生物传感器。通过电化学方法研究了亮丙瑞林（LPR）与固定在铅笔石墨电极（PGE）上的双链 DNA（dsDNA）的相互作用。通过两种不同的相互作用方法（在 PGE 表面和溶液相中）使用差分脉冲伏安法研究并证实了相互作用的机制。在 pH 值为 4.80 的乙酸缓冲液中，使用鸟嘌呤氧化峰电流的减小作为相互作用的指示剂。针对两种相互作用方法，优化了在电极表面和溶液相中进行相互作用的累积时间、电位、药物浓度和重现性。在 DNA 修饰的 PGE 上，在 0.20-6.00 ppm LPR 浓度范围内获得了线性响应，检测限为 0.06 ppm，而在溶液相相互作用方法中，在 0.20-1.00 ppm 浓度范围内检测限为 0.04 ppm。在裸 PGE 上，在所有研究的 pH 值下，LPR 均表现出不可逆的氧化行为。建立了用于测定 LPR 的差分脉冲吸附剥离（AdSDPV）伏安法。在这些条件下，电流在 0.005-0.20 ppm 的范围内与浓度呈线性关系，检测限为 0.0014 ppm。每种测定方法均经过充分验证，并应用于其药物制剂中 LPR 的分析。
