Nishigami T
Department of Ophthalmology, Tottori University School of Medicine, Yonago, Japan.
Nippon Ganka Gakkai Zasshi. 1990 Feb;94(2):128-34.
Sprague-Dawley rats (5 weeks old) were injected intravenously with 65mg of streptozotocin (STZ)/kg body weight. The diabetic rats were injected subcutaneously with insulin chronically or acutely and biochemical parameters in the lens were determined. Increases in aldose reductase (AR) activity and contents of sobitol, glucose and fructose were found in the STZ-diabetic rats, compared with normal control rats. When the STZ-diabetic rats were injected with 10 units of monotard insulin on each of 7 successive days, AR activity tended to decrease, and contents of sorbitol and fructose significantly decreased, but the glucose content did not significantly decrease, compared with STZ-diabetic rats given no insulin. There was no decrease in AR activity in the STZ-diabetic rats 3 hours after an injection of 10 units of actrapid insulin. These results demonstrated that the chronic administration of monotard insulin may decrease AR activity and sorbitol content in the lens of STZ-diabetic rats without lowering glucose levels.
将65毫克链脲佐菌素（STZ）/千克体重静脉注射到5周龄的斯普拉格-道利大鼠体内。对糖尿病大鼠进行慢性或急性皮下胰岛素注射，并测定晶状体中的生化参数。与正常对照大鼠相比，STZ诱导的糖尿病大鼠的醛糖还原酶（AR）活性以及山梨醇、葡萄糖和果糖含量增加。当给STZ诱导的糖尿病大鼠连续7天每天注射10单位中效胰岛素时，与未注射胰岛素的STZ诱导的糖尿病大鼠相比，AR活性趋于降低，山梨醇和果糖含量显著降低，但葡萄糖含量没有显著降低。注射10单位速效胰岛素3小时后，STZ诱导的糖尿病大鼠的AR活性没有降低。这些结果表明，长期给予中效胰岛素可能会降低STZ诱导的糖尿病大鼠晶状体中的AR活性和山梨醇含量，而不会降低血糖水平。
