Cano-Martínez Agustina, Vargas-González Alvaro, Guarner-Lans Verónica, Prado-Zayago Esteban, León-Oleda Martha, Nieto-Lima Betzabé
Departamento de Fisiología, Instituto Nacional de Cardiología Ignacio Chávez.
Arch Cardiol Mex. 2010 Apr-Jun;80(2):79-86.
"In the present study we evaluated the effect of partial ventricular amputation (PVA) in the heart of the adult urodele amphibian (Ambystoma mexicanum) in vivo on spontaneous heart contractile activity recorded in vitro in association to the structural recovery at one, five, 30 and 90 days after injury. One day after PVA, ventricular-tension (VT) (16 ± 3%), atrium-tension (AT) (46 ± 4%) and heart rate (HR) (58+10%) resulted lower in comparison to control hearts. On days five, 30 and 90 after damage, values achieved a 61 ± 5, 93 ± 3, and 98 ± 5% (VT), 60 ± 4, 96 ± 3 and 99 ± 5% (AT) and 74 ± 5, 84 ± 10 and 95 ± 10% (HR) of the control values, respectively. Associated to contractile activity recovery we corroborated a gradual tissue restoration by cardiomyocyte proliferation. Our results represent the first quantitative evidence about the recovery of heart of A. mexicanum restores its functional capacity concomitantly to the structural recovery of the myocardium by proliferation of cardiomyocytes after PVA. These properties make the heart of A. mexicanum a potential model to study the mechanisms underlying heart regeneration in adult vertebrates in vivo.
在本研究中，我们评估了成年有尾两栖动物（墨西哥钝口螈）心脏部分心室切除术（PVA）对体外记录的自发性心脏收缩活动的影响，并将其与损伤后1天、5天、30天和90天的结构恢复相关联。PVA术后1天，与对照心脏相比，心室张力（VT）（16±3%）、心房张力（AT）（46±4%）和心率（HR）（58±10%）降低。在损伤后的第5天、30天和90天，VT值分别达到对照值的61±5%、93±3%和98±5%，AT值分别达到60±4%、96±3%和99±5%，HR值分别达到74±5%、84±10%和95±10%。与收缩活动恢复相关，我们证实了心肌细胞增殖导致组织逐渐恢复。我们的结果首次提供了定量证据，表明墨西哥钝口螈心脏在PVA后通过心肌细胞增殖在结构恢复的同时恢复其功能能力。这些特性使墨西哥钝口螈的心脏成为研究成年脊椎动物体内心脏再生潜在机制的一个潜在模型。
