Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
J Hered. 2011 Jan-Feb;102(1):102-12. doi: 10.1093/jhered/esq105. Epub 2010 Dec 9.
The Asian tiger mosquito, Aedes (Stegomyia) albopictus (Skuse), is an important vector of a number of arboviruses, and populations exhibit extreme variation in adaptive traits such as egg diapause, cold hardiness, and autogeny (ability to mature a batch of eggs without blood feeding). The genetic basis of some of these traits has been established, but lack of a high-resolution linkage map has prevented in-depth genetic analyses of the genes underlying these complex traits. We report here on the breeding of 4 F(1) intercross mapping families and the use of these to locate 35 cDNA markers to the A. albopictus linkage map. The present study increases the number of markers on the A. albopictus cDNA linkage map from 38 to 73 and the density of markers from 1 marker/5.7 cM to 1 marker/2.9 cM and adds 9, 16, and 10 markers to the 3 linkage groups, respectively. The overall lengths of the 3 linkage groups are 64.5, 76.5, and 71.6 cM, respectively, for a combined length of 212.6 cM. Despite conservation in the order of most genes among the 4 families and a previous mapping family, we found substantial heterogeneity in the amount of recombination among markers. This was most marked in linkage group I, which varied between 16.7 and 69.3 cM. A map integrating the results from these 4 families with an earlier cDNA linkage map is presented.
亚洲虎蚊，白纹伊蚊（Stegomyia），是多种虫媒病毒的重要载体，其种群在适应特征方面表现出极端的变异，如卵滞育、耐寒性和自育性（无需吸血即可成熟一批卵的能力）。这些特征中的一些遗传基础已经建立，但缺乏高分辨率的连锁图谱，阻碍了对这些复杂特征相关基因的深入遗传分析。我们在此报告了 4 个 F(1) 杂交作图家系的繁殖情况，并利用这些家系将 35 个 cDNA 标记定位到白纹伊蚊连锁图谱上。本研究将白纹伊蚊 cDNA 连锁图谱上的标记数量从 38 个增加到 73 个，标记密度从 1 个标记/5.7 cM 增加到 1 个标记/2.9 cM，分别在 3 个连锁群上增加了 9、16 和 10 个标记。3 个连锁群的总长度分别为 64.5、76.5 和 71.6 cM，总长度为 212.6 cM。尽管在 4 个家系和之前的一个作图家系中大多数基因的顺序是保守的，但我们发现标记之间的重组数量存在很大的异质性。在连锁群 I 中最为明显，其范围为 16.7 到 69.3 cM。本文呈现了一个整合了这 4 个家系和之前 cDNA 连锁图谱结果的图谱。
