Severson D W, Mori A, Kassner V A, Christensen B M
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
Insect Mol Biol. 1995 Feb;4(1):41-5. doi: 10.1111/j.1365-2583.1995.tb00006.x.
Aedes albopictus and Aedes aegypti are members of the mosquito family Culicidae and share a haploid chromosome complement of three. Although a genetic linkage map based on restriction fragment length polymorphism (RFLP), markers exists for Ae. aegypti, the extent of synteny and linkage order conservation between the two species was unknown. A comparative linkage map for Ae. albopictus was constructed based mainly on cDNA clones from Ae. aegypti. Nearly all Ae. aegypti probes hybridized to Ae. albopictus DNA at high stringency. For eighteen RFLP markers tested, the linkage group and linear order appears to be identical for the two species. 78% of the loci tested exhibited significant deviations from the expected segregation ratio in at least one of the test crosses. An excess of heterozygote genotypes was recovered with most loci. This probably reflects the effects of lethal loci on survival of F2 progeny homozygous for the parental genotypes. These results demonstrate that comparative linkage maps based on common DNA markers provide a basis for rapidly developing linkage maps for various mosquito species, and the opportunity to examine the significance and function of orthologous quantitative trait loci associated with mosquito vector competence for disease transmission.
白纹伊蚊和埃及伊蚊是蚊科库蚊亚科的成员，单倍体染色体组均为三条。虽然已有基于限制性片段长度多态性（RFLP）标记构建的埃及伊蚊遗传连锁图谱，但这两个物种之间的同线性程度和连锁顺序保守性尚不清楚。主要基于埃及伊蚊的cDNA克隆构建了白纹伊蚊的比较连锁图谱。几乎所有埃及伊蚊探针都能与白纹伊蚊DNA在高严谨度下杂交。对于所测试的18个RFLP标记，两个物种的连锁群和线性顺序似乎相同。在至少一个测交中，78%的测试位点表现出与预期分离比的显著偏差。大多数位点回收的杂合子基因型过多。这可能反映了致死位点对亲本基因型纯合的F2代存活的影响。这些结果表明，基于共同DNA标记的比较连锁图谱为快速构建各种蚊种的连锁图谱提供了基础，也为研究与蚊媒疾病传播能力相关的直系同源数量性状位点的意义和功能提供了机会。
