Mandell J W, Townes-Anderson E, Czernik A J, Cameron R, Greengard P, De Camilli P
Department of Physiology, Cornell University Medical College, New York, New York 10021.
Neuron. 1990 Jul;5(1):19-33. doi: 10.1016/0896-6273(90)90030-j.
The vertebrate retina contains two ultrastructurally distinct types of vesicle-containing synapses: conventional synapses, made predominantly by amacrine cells, and ribbon synapses, formed by photoreceptor and bipolar cells. To identify molecular differences between these synapse types, we have compared the distribution of the synapsins, a family of nerve terminal phosphoproteins, with that of synaptophysin (p38) and SV2, two intrinsic membrane proteins of synaptic vesicles. We report an absence of synapsin I and II immunoreactivity from all ribbon-containing nerve terminals. These include terminals of rod cells in developing and adult rat retina, rod and cone cells in monkey and salamander retinas, and rat bipolar cells. Furthermore, we show that synapsins I and II are differentially distributed among conventional synapses of amacrine cells. The absence of the synapsins from ribbon synapses suggests that vesicle clustering and mobilization in these terminals differ from that in conventional synapses.
传统突触，主要由无长突细胞形成；以及带状突触，由光感受器和双极细胞形成。为了确定这些突触类型之间的分子差异，我们比较了突触素（一种神经末梢磷蛋白家族）与突触囊泡蛋白（p38）和SV2（突触囊泡的两种内在膜蛋白）的分布情况。我们报告称，所有含带状结构的神经末梢均不存在突触素I和II免疫反应性。这些包括发育中和成年大鼠视网膜中的视杆细胞末梢、猴和蝾螈视网膜中的视杆和视锥细胞末梢以及大鼠双极细胞末梢。此外，我们表明突触素I和II在无长突细胞的传统突触中分布不同。带状突触中不存在突触素表明这些末梢中的囊泡聚集和动员与传统突触中的不同。
