Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Mycologia. 2004 Mar-Apr;96(2):208-10.
A new method for scanning electron microscopy (SEM) of fungal mitotic nuclei and chromosomes was established for two ascomycetes, Cochliobolus heterostrophus and Neurospora crassa. Nuclei and chromosomes discharged from germling cells by the germ-tube burst method were spread on a surface of a glass slide. The spreads were impregnated with osmium-thiocarbohydrazide for conductive staining, followed by coating with platinum, and observed by field-emission SEM. Ultrastructure of fungal chromosomes and nuclei was visualized by SEM for the first time.
建立了一种用于扫描电子显微镜（SEM）观察两种子囊菌（ Cochliobolus heterostrophus 和 Neurospora crassa ）有丝分裂核和染色体的新方法。通过芽管爆裂法从幼孢子细胞中释放出的核和染色体被铺展在载玻片表面。铺片用锇-硫代碳水化合物进行导电性染色，然后用铂进行涂层，用场发射 SEM 观察。真菌染色体和核的超微结构首次通过 SEM 可视化。
