Gale L R, Bryant J D, Calvo S, Giese H, Katan T, O'Donnell K, Suga H, Taga M, Usgaard T R, Ward T J, Kistler H C
Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, St. Paul, Minnesota 55108, USA.
Genetics. 2005 Nov;171(3):985-1001. doi: 10.1534/genetics.105.044842. Epub 2005 Aug 3.
A genetic map of the filamentous fungus Fusarium graminearum (teleomorph: Gibberella zeae) was constructed to both validate and augment the draft whole-genome sequence assembly of strain PH-1. A mapping population was created from a cross between mutants of the sequenced strain (PH-1, NRRL 31084, originally isolated from Michigan) and a field strain from Minnesota (00-676, NRRL 34097). A total of 111 ascospore progeny were analyzed for segregation at 235 loci. Genetic markers consisted of sequence-tagged sites, primarily detected as dCAPS or CAPS (n = 131) and VNTRs (n = 31), in addition to AFLPs (n = 66) and 7 other markers. While most markers exhibited Mendelian inheritance, segregation distortion was observed for 25 predominantly clustered markers. A linkage map was generated using the Kosambi mapping function, using a LOD threshold value of 3.5. Nine linkage groups were detected, covering 1234 cM and anchoring 99.83% of the draft sequence assembly. The nine linkage groups and the 22 anchored scaffolds from the sequence assembly could be assembled into four chromosomes, leaving only five smaller scaffolds (59,630 bp total) of the nuclear DNA unanchored. A chromosome number of four was confirmed by cytological karyotyping. Further analysis of the genetic map data identified variation in recombination rate in different genomic regions that often spanned several hundred kilobases.
构建了禾谷镰刀菌（有性型：玉蜀黍赤霉）的遗传图谱，以验证和完善菌株PH-1的全基因组序列草图组装。通过对已测序菌株（PH-1，NRRL 31084，最初从密歇根州分离）的突变体与明尼苏达州的一个田间菌株（00-676，NRRL 34097）进行杂交，创建了一个作图群体。总共对111个四分体子囊孢子后代进行了235个位点的分离分析。遗传标记包括序列标签位点，主要检测为dCAPS或CAPS（n = 131）和VNTRs（n = 31），此外还有AFLPs（n = 66）和其他7个标记。虽然大多数标记表现出孟德尔遗传，但观察到25个主要成簇的标记存在分离畸变。使用Kosambi作图函数生成了一个连锁图谱，LOD阈值为3.5。检测到九个连锁群，覆盖1234 cM，锚定了99.83%的序列组装草图。这九个连锁群和序列组装中的22个锚定支架可以组装成四条染色体，仅留下核DNA的五个较小支架（共59,630 bp）未锚定。通过细胞学核型分析确认染色体数目为四条。对遗传图谱数据的进一步分析确定了不同基因组区域中重组率的变化，这些区域通常跨越数百千碱基。
